Figure 3.

Cytotoxicity and intracellular uptake of Carex in H9C2 cardiomyoblast. (A) H9C2 cells were supplemented with Carex, and cell viability was assessed at 0, 48, 72, and 96 h. Note that no cytotoxicity was observed regardless of the Carex dosages. (B,C) Fluorescence microscopic analysis (B) and flow cytometry (C) of intracellular delivery of Carex. Carex was stained with PKH67 dye (B) or DiI dye (C) and supplemented to the culture medium. Cells were further cultured for 4 and 6 h for fluorescence microscopic analysis and 4 h for flow cytometric analysis. The uptake rate (%) was calculated from the flow cytometric analysis results. All values are expressed as mean ± SD (* p < 0.05, NS: not significant; n = 3).