Extended Data Table 2. Penetrances of the Tex phenotype produced in wild-type animals by RNAi against cell-cycle genes with potential roles in cell extrusion.
The Tex penetrance produced in wild-type animals by RNAi clones against cell cycle genes that might be involved in cell extrusion (based on the corresponding Tex penetrance in ced-3(lf) animals) is provided. Bona fide candidates for cell extrusion regulation should not produce a Tex phenotype in wild-type animals, as cell extrusion does not occur in wild-type embryos. A Tex phenotype in wild-type animals could occur from other effects of RNAi against cell cycle genes, such as excessive proliferation leading to multiple excretory cells. Such proliferation is likely the case for lin-23, as RNAi against lin-23 has been previously described to cause excessive proliferation51. The 13 other genes are good candidates to be regulators of cell extrusion by the criterion of dependence of the Tex phenotype on the loss of function of ced-3.
| RNAi target | Mammalian Homolog | %Tex | n |
|---|---|---|---|
| empty vector | - | 0 | 127 |
| atl-1 | ATR | 0 | 198 |
| cdc-25.2 | CDC25 | 0 | 36 |
| cdk-1 | CDK1 | 0 | 51 |
| cdk-2 | CDK2 | 0 | 237 |
| chk-1 | CHK1 | 0 | 115 |
| csn-1 | GPS1 | 0 | 156 |
| csn-4 | COPS4 | 0 | 141 |
| csn-5 | COPS5 | 0 | 114 |
| cya-1 | CCNA | 0 | 167 |
| cye-1 | CCNE | 0 | 143 |
| lin-23 | βTrCP | 12 | 96 |
| psf-1 | GINS1 | 0 | 150 |
| psf-2 | GINS2 | 0 | 190 |
| psf-3 | GINS3 | 0 | 72 |