Table 1. Primers used in the study.
Primer name | Primer sequences | Tm (°C) | Length (bp) | Ec (%) |
---|---|---|---|---|
DP-SOD-F | AATGCTGCGTTCTCTGTTGTG | 58.7 | 335 | |
DP-SOD-R | TCTGGTTTTGTTGTTTCAGGAGT | 58.4 | ||
DP-POD-F | CAACCCCGACCAGCCCTAC | 62.3 | 600 | |
DP-POD-R | AAAAGGGGAAATCGGTGTCG | 61.4 | ||
DP-GST-F | TGACCGTGAACCAGACCGAG | 61.3 | 431 | |
DP-GST-R | GATGCCGAAAATACTGAGTGTGG | 61.4 | ||
qPCR-SOD-F | GAAATAACTGGTTCCAAGGCACT | 59.6 | 125 | 91.8 |
qPCR-SOD-R | AATGCTGCGTTCTCTGTTGTG | 58.7 | ||
qPCR-POD-F | CCGCACTGGGACGACGAGAC | 65.8 | 235 | 96.4 |
qPCR-POD-R | CGATGAGCGAGTGGAAGTATCTGAA | 64.8 | ||
qPCR-GST-F | GCTGCTGCTGTGCTGGATTA | 59.7 | 170 | 90.0 |
qPCR-GST-R | ACCGTGAACCAGACCGAGAC | 59.4 | ||
EF-1-F | TCAAGGAACTGCGTCGTGGAT | 58.6 | 130 | 95.4 |
EF-1-R | ACAGGGGTGTAGCCGTTAGAG | |||
18S-F | AACACGGGAAACCTCACCA | 55.4 | 116 | 108.9 |
18S-R | CAGACAAATCGCTCCACCAA | |||
RPL32-F | CAACATCGGTTATGGAAGCA | 55.0 | 141 | 100.1 |
RPL32-R | ACAGCGTGGGCAATTTCAGC | |||
GAPDH-F | AAGGGTGCTCAGGTTGTTGCT | 56.5 | 89 | 104.4 |
GAPDH-R | CGACCGTGGGTGGAGTCATAT |