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. Author manuscript; available in PMC: 2022 Feb 11.
Published in final edited form as: Nature. 2021 Aug 11;596(7873):603–607. doi: 10.1038/s41586-021-03803-w

Extended Data Figure 7 related to Figure 3. Cryo-EM single particle reconstruction of the holo L-16 ScaI Tetrahymena ribozyme.

Extended Data Figure 7 related to Figure 3.

(a) Single particle pipeline yields the final cryo-EM reconstruction with the corresponding angular distribution and local resolution map. The local resolution map shows more flexibility and lower resolution in the 4-nt bulge that connects stem P2.1 and P13, and toward the end of stem P6 and P9.2. (b) FSC curve shows 3.1 Å resolution according to the 0.143 cutoff. (c) Cryo-EM B-factor.