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. Author manuscript; available in PMC: 2022 Feb 11.
Published in final edited form as: Nature. 2021 Aug 11;596(7873):603–607. doi: 10.1038/s41586-021-03803-w

Figure 2. Novel structural insights in the peripheral regions of the apo L-21 ScaI ribozyme.

Figure 2.

(a) Base triple consists of U43 and A171-A172 A-platform that connects P5c with P14. (b) A46 in P2 and A210 in P4 forms a ‘P2-P4-P14’ bridge (c) P14 that coaxially stacks with P5c and P2. (d) P2 forms a A31-U56-A95 base triple and connects with P2.1. (e) The A31-U56-A95 base triple. (f) G358 interacts with C266-G309-A306 to form a base quartet. (g) The bulge region in stem P9.1 stabilizes the catalytic site stem P7 via a purine-minor interaction. (h) G358 and A359 interacts with A265-U310-A261 to form base quartets. (i) G360 interacts with G264-C311 to form a base triple. Newly resolved peripheral region is presented with the cryo-EM map visualized at 3σ threshold. The cryo-EM local regions for all subpanels are visualized at 1σ threshold except for (c) at 2σ, (d and g) at 1.5σ. Dashed line indicates hydrogen bond.