FIG 2.
A loss-of-function (LoF) genetic screen identifies genes involved in antimicrobial activity. (A) Workflow of the LoF genetic screen. (B) Genomic locations of BfN transposon insertions that abrogate its capacity to inhibit growth of BfH610. (C) Quantification of killing activity by BfN parental and transposon mutant strains identified in panel B. (D) Quantification of killing activity by BfN parental, isogenic deletion strains, and complemented mutants. Complemented mutants carry the deleted gene in single copy in a neutral locus (att). For panels C and D, mean number of target strain input CFU is indicated with a gray line (mean = 2.2 × 107); line width represents SD (SD = 8.5 × 106). Target strain output CFU are reported. Error bars indicate SD (n = 2; representative of three independent experiments). Different letters represent groups that are statistically significantly different (P < 0.05).