FIG 3.
Activity of Fpn homologs from BfN and susceptible target strains. (A) Western blot analysis of BFT products generated following incubation of purified BfN Fpn or its catalytic residue mutants (FpnH135A; FpnC180A) with BFT carrying a C-terminal c-Myc tag. (B) Mutation of Fpn catalytic residues in BfN abrogates its ability to inhibit BfR growth. The mean number of target strain input CFU is indicated with a gray line (mean = 6.0 × 107); line width represents SD (SD = 5.0 × 106). Target strain output CFU are reported. Error bars indicate SD (n = 2; representative of three independent experiments). (C) Expression of fpn genes derived from susceptible strains BfH610 and BfH615 restores killing activity to a BfNΔfpn mutant. The mean number of target strain input CFU is indicated with a gray line (mean = 9.6 × 105); line width represents SD (SD = 2.4 × 105). Target strain output CFU are reported. Error bars indicate SD (n = 2; representative of three independent experiments). For panels B and C, different letters represent groups that are statistically significantly different (P < 0.05).