Abstract
GUDDING, ROAR: The suitability of some media and peptones for sulfonamide testing. Acta vet. scand. 1974, 15, 366–380. — The content of p-aminobenzoic acid and folic acid has been determined in some media and peptones, and the sensitivity of Bacillus megaterium, cultivated on these substrates, towards sulfonamides has been examined.
The concentrations of p-aminobenzoic acid and folic acid should be as low as possible in suitable substrates, but other factors are also of importance. The presence of antagonists towards folic acid has been demonstrated in some media, and the consequences of these inhibitors are discussed.
Three of the media (Paper Disc Method-Antibiotic Sensitivity Medium, AB Biodisk; Sulphonamide Antagonist Free Medium, Mast Laboratories; and Mueller Hinton, Difco Laboratories) seem to be satisfactory for the determination of the resistance of bacteria towards sulfonamides. The application of Acetobacter medium is suggested for the detection of rest concentrations of sulfonamides in foodstuffs.
detection of sulfonamide residues; p-aminobenzoic acid; folic acid.
Sammanfattning
Brukbarheten av enkelte medier og peptoner til undersøkelse av sulfonamider.
Innholdet av p-aminobenzoesyre og folinsyre er blitt undersøkt i enkelte medier og peptoner, og følsomheten av Bacillus megaterium dyrket på disse substratene overfor sulfonamider er blitt undersøkt.
Lavest mulige konsentrasjoner av p-aminobenzoesyre og folinsyre er vesentlig for brukbarheten av de enkelte substratene, men andre faktorer virker også inn. Det er vist at enkelte medier inneholder antagonister overfor folinsyre, og betydningen av slike inhibitorer er diskutert.
Tre av de undersøkte mediene (Paper Disc Method-Antibiotic Sensitivity Medium, AB Biodisk; Sulphonamide Antagonist Free Medium. Mast Laboratories; og Mueller Hinton, Difco Laboratories) synes å være tilfredsstillende ved resistensbestemmelse av bakterier overfor sulfonamider. Acetobacter medium er foreslått benyttet ved påvisning av restkonsentrasjoner av sulfonamider i næringsmidler.
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Footnotes
This investigation was supported by the Agricultural Research Council of Norway.
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