Skip to main content
NCBI home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1974 Mar 1;15(1):90–99. doi: 10.1186/BF03547496

Diurnal Variation of Peripheral Plasma Levels of Testosterone in Bulls Measured by a Rapid Radioimmunoassay Procedure

Dygnsvariationen av blodplasmanivåer av testosteron hos tjur mätt med en radioimmunologisk metod

Prakash Chandra Sanwal 1, Anne Sundby 1, Lars-Eric Edqvist 1,✉
PMCID: PMC8407176  PMID: 4830073

Abstract

A radioimmunoassay technique, using antibodies to testosterone-3-(O-carboxymethyl) oxime-bovine serum albumin, has been applied for the measurement of peripheral plasma levels of testosterone in bulls. The individual as well as the mean 24-hr. pattern of peripheral plasma levels of testosterone in the four bulls investigated showed three distinct peaks. The mean peripheral plasma level of testosterone peaked around 10 p.m., around 6 a.m. and around noon, the mean levels obtained at these times being 6.2, 6.1 and 4.2 ng/ml, respectively. In one bull an additional peak occurred around 2 a.m. (8.2 ng/ml). Significantly lower peripheral plasma levels of testosterone were observed between the peak values.

Keywords: testosterone, blood, radioimmunoassay, bulls

Full Text

The Full Text of this article is available as a PDF (984.5 KB).

Footnotes

This study was supported by the Agricultural Research Council of Norway and the Swedish Council for Forestry and Agricultural Research

References

  1. Anderson D C. A simple and clinically useful method for plasma testosterone-like substances by competitive protein binding. Clin. chim. Acta. 1970;29:513–522. doi: 10.1016/0009-8981(70)90023-9. [DOI] [PubMed] [Google Scholar]
  2. Coyotupa J, Parlow A F, Abraham G E. Simultaneous radioimmunoassay of plasma testosterone and dihydrotestosterone. Analyt. Letters. 1972;5:329–340. doi: 10.1080/00032717208066108. [DOI] [Google Scholar]
  3. Edqvist, L. E. & E. D. B. Johansson: Radioimmunoassay of oestrone and oestradiol in human and bovine peripheral plasma. Acta endocr. (Kbh.) 1972a, 71, 716–730. [DOI] [PubMed]
  4. Edqvist, L. E. & E. D. B. Johansson: Radioimmunoassay and competitive protein binding for the measurement of certain steroid hormones in farm animals. In Isotope Studies on the Physiology of Domestic Animals, IAEA, Vienna 1972b, 245–258.
  5. Erlanger B F, Borek F, Beiser S, Liebermann S. Preparation and characterization of conjugates of bovine serum albumin with testosterone and with cortisone. J. biol. Chem. 1958;228:713–727. [PubMed] [Google Scholar]
  6. Erlanger, B. F., S. M. Beiser, F. Borek, F. Edel & S. Liebermann: In Methods in Immunology and Immunochemistry. Williams, C. A. and M. G. Chase, eds, Acad. Press Inc., New York 1967, Vol. I, pp. 144 and 151.
  7. Furuyama S, Mayes D M, Nugent C A. A radioimmunoassay for plasma testosterone. Steroids. 1970;16:415–428. doi: 10.1016/S0039-128X(70)80124-6. [DOI] [PubMed] [Google Scholar]
  8. Katongole C B, Naftolin F, Short R Y. Relationship between blood levels of luteinizing hormone and testosterone in bulls, and the effect of sexual stimulation. J. Endocr. 1971;50:457–466. doi: 10.1677/joe.0.0500457. [DOI] [PubMed] [Google Scholar]
  9. Martinsson K, Lindberg P, Johansson E D B. Preparation of protein-steroid conjugates and antisera against oestradiol-17β. Acta vet. scand. 1973;14:278–291. doi: 10.1186/BF03547446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Thorneycroft, I. H., S. A. Tillson, G. E. Abraham, R. J. Scaramuzzi & B. V. Caldwell: In Immunologic Methods in Steroid Determination. Peron, F. G. and B. Y. Caldwell, eds. Appleton-Century-Crofts, Education Division, Meredith Corporation, New York 1970, p. 63.
  11. Vermeulen, A. & L. Verdonck: Testosterone assays by competitive protein binding. Acta endocr. (Kbh.) 1970, Suppl. No. 147, 239–256. [DOI] [PubMed]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES