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. Author manuscript; available in PMC: 2022 Aug 24.
Published in final edited form as: Biochemistry. 2021 Aug 6;60(33):2524–2536. doi: 10.1021/acs.biochem.1c00384

Table 1.

Steady-state kinetic parameters of KDAC8 with FRKacRW in buffers of varying ionic strength

Reaction buffer Specific activity
(s−1)		 KM
(μM)		 kcat
(s−1)		 kcat/KM
(M−1 s−1)
High ionic strength* 0.014 ± 0.003 950 ± 110 0.162 ± 0.007 170 ± 13
Low ionic strength 0.035 ± 0.007† 500 ± 50† 0.233 ± 0.006† 470 ± 40†
*

Previously reported.22

†

Statistically significant difference from the high ionic strength buffer.