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. 2021 Feb 23;35(9):2658–2671. doi: 10.1038/s41375-021-01158-9

Fig. 5. G-CSF and AMD3100 mobilization in caspase-1-KO and Nlrp3-KO animals is not restored by supplementation with IL-1β + IL-18 but requires the presence of DAMPs.

Fig. 5

A WBCs (left), SKL cells (middle), and CFU-GM clonogenic progenitors (right) were obtained from WT mice injected with PBS or G-CSF (180 µg/kg) or Casp1-KO mice injected with G-CSF with or without a mixture of IL-1β and IL-18 (0.5 µg each per mouse) or a DAMP cocktail (ATP [3 mg/kg] + HMGB1 [1.5 μg] + S100A9 [2 μg] per mouse). B WBCs (left), SKL cells (middle), and CFU-GM clonogenic progenitors (right) were obtained from WT mice injected with PBS or AMD3100 (5 mg/kg) or Casp1-KO mice injected with AMD3100 with or without a mixture of IL-1β and IL-18 (0.5 µg each per mouse) or a DAMP cocktail (ATP [3 mg/kg] + HMGB1 [1.5 μg] + S100A9 [2 μg] per mouse). AMD3100 was injected once, whereas G-CSF was administered on 4 consecutive days. AMD3100-injected mice were sacrificed 1 h post injection, whereas G-CSF-treated animals were sacrificed 6 h after the last injection. The number of SKL cells and CFU-GM progenitors mobilized into PB was calculated using the formula described in Materials and Methods. The data are presented as means ± SE, and an unpaired Student’s t-test was used for the determination of significance (*p ≤ 0.05 and #p ≤ 0.005). C WBCs (left), SKL cells (middle), and CFU-GM clonogenic progenitors (right) were obtained from WT mice injected with PBS or G-CSF (5 mg/kg) or Nlrp3-KO mice injected with G-CSF with or without a mixture of IL-1β and IL-18 (0.5 µg each per mouse) or a DAMP cocktail (ATP [3 mg/kg] + HMGB1 [1.5 μg] + S100A9 [2 μg] per mouse). D WBCs (left), SKL cells (middle), and CFU-GM clonogenic progenitors (right) obtained from WT mice injected with PBS or AMD3100 (5 mg/kg) or Nlrp3-KO mice injected with AMD3100 (5 mg/kg) with or without a mixture of IL-1β and IL-18 (0.5 µg each per mouse) or a DAMP cocktail (ATP [3 mg/kg] + HMGB1 [1.5 μg] + S100A9 [2 μg] per mouse). AMD3100 was injected once, whereas G-CSF was administered on 4 consecutive days. AMD3100-injected mice were sacrificed 1 h post injection, whereas G-CSF-treated animals were sacrificed 6 h after the last injection. The numbers of SKL cells and CFU-GM progenitors mobilized into PB were calculated using the formula described in Materials and methods. The data are presented as means ± SE, and an unpaired Student’s t-test was used for the determination of significance (*p ≤ 0.05; #p ≤ 0.005).