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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Neuropharmacology. 2021 Jun 18;195:108673. doi: 10.1016/j.neuropharm.2021.108673

Fig. 1. Concentration-response curves of MOR with graded concentrations of MS1 and Comp5 using L-methadone, morphine, and oxycodone as orthosteric ligands.

Fig. 1.

GαoA dissociation BRET assay of MS1 (A) and Comp5 (B) and β-arrestin recruitment BRET assays of MS1 (C) and Comp5 (D) were performed to assess MS1 and Comp5 allosteric regulation of L-methadone, morphine and oxycodone, respectively, through MOR. Data are mean +/− SEM of a dataset composed of three independent experiments, each one carried out in duplicate. KB is the PAM binding affinity to free receptor, α is the binding cooperativity factor, and β is the efficacy cooperativity factor.