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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Neuropharmacology. 2021 Jun 18;195:108673. doi: 10.1016/j.neuropharm.2021.108673

Fig.2. Effects of MS1 and Comp5 on antinociceptive responses to oxycodone, methadone, and morphine in the hot plate assay.

Fig.2.

Male mice were injected with MS1, Comp5, or vehicle immediately after hot plate baseline assessment, received either opioid or saline treatment 30 min later, and they were tested in the hot plate apparatus 30 min after the last injection. Data are plotted as % maximal possible effect (MPE). (A) MS1 promotes analgesic responses to oxycodone [F(7,40)=92.54, p<0.0001, (n=5-7), ***p=0.0002 for oxycodone 3.5 mg/kg +Veh vs. oxycodone 3.5 mg/kg + MS1. (B) MS1 promotes analgesic responses to methadone [F(7,42)=10.30, p<0.0001, (n=5-9 per group), *p=0.0288 for methadone 5 mg/kg +Veh vs. methadone 5 mg/kg + MS1]. (C) MS1 does not have any effect on the antinociceptive actions of morphine (n=4-18 per group). (D) Comp5 promotes the analgesic responses to oxycodone [F(9,68)= 26.52, p<0.0001, (n=6-12 per group), *p=0.0117 for oxycodone 3.5 mg/kg +Veh vs. oxycodone 3.5 mg/kg + Comp5]. (E) Comp5 has no effect on the actions of methadone (n=5-11). (F) Comp5 increases the efficacy of morphine F(7,63)= 35.43, p<0.0001, (n=5-17), *p=0.0318 for morphine 10 mg/kg +Veh vs. morphine 10 mg/kg + Comp5]. Data are reported as ± SEM. Statistics based on multiple comparisons one-way ANOVA followed by Holm-Sidak’s post hoc test. Morph (Morphine), Oxy (Oxycodone), Veh (Vehicle), and Comp5 (Compound-5). MS1, Comp5, or vehicle were injected i.p. and all opioids were injected s.c.