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. 2021 Aug 28;11(18):9162–9176. doi: 10.7150/thno.59605

Figure 5.

Figure 5

PRMT5 regulated STAT1 transcription through H3R2me2s. Effect of PRMT5 on STAT1 (A) and PD-L1 (B) transcription was analyzed through dual-luciferase reporter assays. (C) The primer design scheme for the STAT1 promoter and its fragment in the ChIP assay. (D-E) Enrichment of PRMT5, H3R2me2s or IgG at the STAT1 promoter was assessed by ChIP Q-PCR. (F) The primer design scheme for the PD-L1 promoter and its fragment in the ChIP assay. (G-H) Enrichment of PRMT5, H3R2me2s or IgG at the PD-L1 promoter was assessed by ChIP Q-PCR. Data are representative of at least two independent experiments. Values are presented as the mean ± SEM. *P < 0.05, **P < 0.01, and *** P < 0.001.