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. 2021 Aug 27;11(18):9118–9132. doi: 10.7150/thno.60132

Figure 7.

Figure 7

Non-invasive [99mTc]-PBT-Fc-direct SPECT revealed renal lesions from chronic hypertension. A. In a chronic hypertensive disease model of RenTg/MK with RAAS over-activation, the eight months old mouse displayed the loss of the tracer signal in regions of the kidney as shown in SPECT (left panels. Top: 3D composite; bottom: section view). Distribution of tracer signal was highly discontinuous. IHC detected fragmented distribution of PBT-Fc (in brown) in whole-kidney (top right) and close-up (bottom right) views. Heatmap scale in %ID/cm3. B. Masson trichrome staining (TrB: fibrosis) of the kidney sections showed lesions consistent with those of chronic hypertensive injury to the kidney, including onion peels-like fibrosis surround the renal arteries, tubular protein casts (arrow), and extensive inflammatory infiltration. Tissue fibrosis was semi-quantified from 10 randomly chosen images in each group. ***, p < 0.001. C. Acute intravenous injection of a mouse with a bolus dose of angiotensin II (Ang II) did not incur the same renal damage as in the chronic hypertensive model in A.