Abstract
Immunomodulatory drugs (IMiDs) are important for the treatment of multiple myeloma and myelodysplastic syndrome. Binding of IMiDs to Cereblon (CRBN), the substrate receptor of the CRL4CRBN E3 ubiquitin ligase, induces cancer cell death by targeting key neo-substrates for degradation. Despite this clinical significance, the physiological regulation of CRBN remains largely unknown. Herein we demonstrate that Wnt, the extracellular ligand of an essential signal transduction pathway, promotes the CRBN-dependent degradation of a subset of proteins. These substrates include Casein kinase 1α (CK1α), a negative regulator of Wnt signaling that functions as a key component of the β-Catenin destruction complex. Wnt stimulation induces the interaction of CRBN with CK1α and its resultant ubiquitination, and in contrast with previous reports does so in the absence of an IMiD. Mechanistically, the destruction complex is critical in maintaining CK1α stability in the absence of Wnt, and in recruiting CRBN to target CK1α for degradation in response to Wnt. CRBN is required for physiological Wnt signaling, as modulation of CRBN in zebrafish and Drosophila yields Wnt-driven phenotypes. These studies demonstrate an IMiD-independent, Wnt-driven mechanism of CRBN regulation and provide a means of controlling Wnt pathway activity by CRBN, with relevance for development and disease.
Subject terms: Morphogen signalling, Ubiquitin ligases, Ubiquitylation
Cereblon (CRBN) is an E3 ubiquitin ligase substrate receptor that is involved in cancer cell death, although its regulation is poorly understood. Here, the authors show that Wnt ligand increases CRBN-dependent protein degradation and demonstrate CRBN’s importance in physiological Wnt signaling.
Introduction
The Wnt signaling pathway is one of the major signal transduction cascades that regulate metazoan development and control tissue homeostasis. Consistent with these essential physiological roles, deregulated Wnt signaling is a driver of many human disease states1. In the absence of Wnt ligands, the pivotal transcriptional coactivator β-Catenin is targeted for proteasomal degradation via its association with a macromolecular protein complex termed the destruction complex2–6. This complex includes the Wnt regulators Adenomatous polyposis coli (APC)3, Axin4,5, Glycogen synthase kinase 3 (GSK3)4,6, and Casein kinase 1α (CK1α)5,6. Upon Wnt stimulation, the degradation of β-Catenin via the destruction complex is attenuated7,8, allowing β-Catenin to accumulate in the nucleus and regulate the transcription of specific target genes9,10. Consistent with its essential role in the destruction complex, CK1α has been identified as one of the crucial negative regulators of Wnt signaling11,12. Although we and others have reported that CK1α is important to this process during metazoan development and in disease settings13–16, little is known about how CK1α itself is regulated in cells.
A number of small molecules have been shown to control the activity of CK1α14–21, amongst which the immunomodulatory drug (IMiD) lenalidomide induces CK1α protein degradation18,21. This process is mediated by a macromolecular E3 ubiquitin ligase complex consisting of the ligase scaffold Cullin-4 (CUL4), the RING-finger protein RING-box1 (RBX1), the adapter Damage-specific DNA binding protein 1 (DDB1), and the substrate receptor Cereblon (CRBN) (CRL4CRBN)18,21–23. Thus, when bound to such IMiDs, CRBN is able to target a number of neo-substrates for degradation, including Ikaros and CK1α, thereby inhibiting cancer growth18,24–31. Despite this established role of CRBN as an anticancer drug target, its physiological role remains poorly understood. Here, we demonstrate a small molecule-independent, evolutionarily conserved mechanism in which Wnt pathway activation stimulates CRBN to associate with CK1α and mediate ubiquitin-dependent CK1α degradation. This mechanism further modulates Wnt signal transduction, with importance for development and disease.
Results
Wnt stimulation induces ubiquitin-dependent, proteasomal degradation of CK1α
Based on our previous work15, which showed reduced levels of CK1α in APC mutant cells, we postulated that Wnt signaling might regulate CK1α protein levels. To test this, we treated HEK293T (HEK) cells with recombinant Wnt3a for varying lengths of time or concentrations and analyzed CK1α levels in cell lysates. Wnt3a treatment reduced CK1α levels in both a time- and concentration-dependent manner, relative to the control protein α-Tubulin (Fig. 1a and Supplementary Fig. 1a). We also noted that recombinant Frizzled8 cysteine-rich domain (FZD8-CRD)32, a Wnt pathway antagonist, blocked the decrease of CK1α levels in response to Wnt3a (Supplementary Fig. 1b), consistent with Wnt3a acting in a specific manner. By contrast, CK1α gene expression was unaffected by Wnt exposure, relative to a Wnt target gene control (Fig. 1b), suggesting that Wnt downregulates CK1α protein levels via a posttranscriptional mechanism. Furthermore, we blocked new protein synthesis in HEK cells using cycloheximide and observed a substantially decreased CK1α half-life in response to Wnt3a (Fig. 1c and Supplementary Fig. 1c). Consistent with Wnt regulating the stability of only a subset of CK1α, the nuclear pool of CK1α was unaffected by Wnt treatment while its corresponding cytoplasmic pool was simultaneously degraded (Supplementary Fig. 1e). Further, unlike the general pool of cytoplasmic CK1α, CK1α associated with the Phosphatase and tensin homolog (PTEN)33 was not degraded in response to Wnt (Supplementary Fig. 1f). Taken together, these results show that Wnt signaling decreases the steady-state levels of a cytoplasmic pool of CK1α by promoting its turnover.
To determine if CK1α levels were regulated via a proteasome- or lysosome/autophagosome-mediated manner we stimulated HEK cells with Wnt3a in the presence of a proteasome (MG132) or lysosome/autophagosome (bafilomycin A1 (BA)) inhibitor. MG132 treatment prevented Wnt3a-dependent CK1α degradation (Fig. 1d), while BA treatment did not (Fig. 1e), suggesting that Wnt-dependent CK1α degradation occurs via the proteasome. To determine if CK1α is ubiquitinated in response to Wnt exposure we treated HEK cells with Wnt3a and then immunoprecipitated CK1α from these cell lysates and immunoblotted for ubiquitin. Wnt3a treatment induced significant levels of CK1α ubiquitination (Fig. 1f). Similar results were obtained using a tandem ubiquitin-binding entity (TUBE) assay (Supplementary Fig. 1d). These results demonstrate that Wnt stimulation results in increased levels of CK1α ubiquitination and subsequent degradation via the proteasome.
Wnt-induced degradation of CK1α requires CRBN, the substrate receptor of the CRL4CRBN E3 ubiquitin ligase complex
We noted that a pan-inhibitor of the RING-finger family of E3 ubiquitin ligases, MLN4924, attenuated Wnt3a-induced CK1α degradation (Supplementary Fig. 2a). We, therefore, evaluated several candidate RING-finger E3 ubiquitin ligases previously implicated in CK1α or Wnt regulation18,34, knocking down their expression in HEK cells and examining CK1α levels (Supplementary Fig. 2b). Interestingly, depletion of CRBN35,36, the substrate receptor of the CRL4CRBN E3 ubiquitin ligase complex, attenuated Wnt-induced CK1α degradation (Fig. 2a and Supplementary Fig. 2b–d). Furthermore, knockdown of CRBN expression prevented the Wnt-induced decrease in CK1α half-life (Fig. 2b and Supplementary Fig. 2e–g), suggesting that the CRL4CRBN E3 ubiquitin ligase complex regulates Wnt-driven CK1α degradation.
To better understand how Wnt induces CRBN-dependent degradation of CK1α, we performed an in vitro binding assay between purified CRBN and recombinant CK1α (Fig. 2c). CRBN was only associated with recombinant CK1α when it was isolated from Wnt-treated cells (Fig. 2d). Notably, CK1α is known to interact with CRBN in a manner dependent on some IMiDs21. Thus, the Wnt-dependent association of CRBN with CK1α is to a point consistent with this previous report, suggesting that the two proteins may require an additional factor/modification to associate upon Wnt stimulation21. We next took advantage of these purified proteins to perform an in vitro CK1α ubiquitination assay, directly determining if Wnt signaling activates CRBN-dependent ubiquitination (Fig. 2c). We found that ubiquitination of recombinant CK1α was significantly enhanced when CRBN was isolated from Wnt-treated cells versus control cells (Fig. 2e). These results suggest that Wnt signaling induces CRBN to bind to and mediate the ubiquitination of CK1α.
The β-Catenin destruction complex regulates CRBN-mediated CK1α degradation
As CK1α is an essential component of the β-Catenin destruction complex5,6, we next determined the kinetics of Wnt-driven CK1α degradation relative to that of other components in the complex. Wnt3a-induced CK1α degradation occurred prior to alterations in the levels of other destruction complex proteins (Fig. 3a and Supplementary Fig. 3a). Importantly, in contrast to CK1α, any Wnt-driven decreases in the levels of other destruction complex components occurred at a later time point (24 h) and were CRBN-independent (Fig. 3b). These results suggest that Wnt-driven, CRBN-dependent CK1α degradation occurs via an intact, functional destruction complex. Based on this finding, we speculated that CRBN is recruited to the destruction complex in response to Wnt stimulation in order to target CK1α. To test this hypothesis, we immunoprecipitated endogenous CRBN from lysates of HEK cells, treated with or without Wnt3a, and probed these immunoprecipitates for components of the destruction complex. We found that CRBN did indeed associate with CK1α and other components of the destruction complex in response to Wnt3a, including the two pivotal scaffolding proteins Axin1 and APC (Fig. 3c). This result suggests that the destruction complex facilitates CRBN-dependent CK1α proteolysis upon Wnt stimulation.
Consistent with the destruction complex stabilizing CK1α in the absence of Wnt stimulation, knockdown of AXIN1 or APC expression induced CK1α degradation (Fig. 3d). Importantly, CK1α degradation following AXIN1 or APC depletion was also CRBN-dependent (Fig. 3d). In cells deficient for AXIN1 or APC, CK1α stabilization resulting from CRBN knockdown did not attenuate β-Catenin stabilization or downstream Wnt activity biomarkers (c-Myc). This observation is consistent with previous work demonstrating the importance of these proteins in efficiently targeting CK1α to β-Catenin5,37. Together, these results suggest that the integrity of the β-Catenin destruction complex is important for the regulation of CRBN-mediated CK1α proteolysis; the destruction complex both stabilizes CK1α in the absence of Wnt and promotes an activation step (via CRBN recruitment) upon Wnt induction.
Wnt stimulation modulates the interaction of CRBN with CK1α in a manner that requires the IMiD binding pocket
CRBN was reported previously to interact with and mediate CK1α ubiquitination only when the IMiD, lenalidomide, formed a molecular bridge between them18,21. This lenalidomide-induced, CRBN-dependent degradation of CK1α occurred via a β-hairpin loop anchored by Gly4021 (Fig. 4a, residues labeled magenta with white text). Similarly, using mutagenesis of key residues, we found that this β-hairpin loop of CK1α is also important for its lenalidomide-independent, Wnt-driven proteolysis (Fig. 4b). We therefore further investigated the role of the IMiD binding pocket of CRBN (Fig. 4a, binding pocket in blue and pivotal CRBN residues labeled green with white text) in Wnt-induced CK1α proteolysis. We mutated five pivotal CRBN residues within this pocket, all of which significantly attenuated their ability to degrade and bind to CK1α (Fig. 4c, d). Thus, Wnt signaling induces CRBN-dependent CK1α degradation in a manner that requires its previously described IMiD binding pocket21.
Due to primary sequence differences between human and mouse CRBN, lenalidomide is not able to induce CK1α degradation in mouse cells18. Thus, we determined whether Wnt-induced CK1α degradation also exhibits this specificity. We compared Wnt3a- and lenalidomide-induced degradation of CK1α in mouse fibroblasts (NIH3T3 cells) and found that Wnt3a-induced CK1α degradation and its binding to CRBN in these mouse cells, whereas lenalidomide did not (Supplementary Fig. 4a, b). This is consistent with a conserved function of Wnt signaling in CRBN-mediated CK1α proteolysis, unlike that of lenalidomide.
Wnt signaling induces CRBN-mediated degradation of a broad spectrum of endogenous substrates
We next determined the ability of Wnt3a to induce the degradation of a broader subset of known, endogenous CRBN substrates, including Glutamine synthetase (GS)38, c-Jun39, and Meis homeobox 2 (MEIS2)40. We noted that in addition to causing decreased levels of CK1α, Wnt3a treatment also resulted in a decrease of the protein levels of these other CRBN substrates (Fig. 5a and Supplementary Fig. 5a), without affecting their levels of gene expression (Supplementary Fig. 5b–e). Similar to CK1α, the half-life of GS, c-Jun, and MEIS2 were also significantly decreased in response to Wnt3a, and this reduction was CRBN-dependent (Fig. 5b–g and Supplementary Fig. 5f–g). In contrast to CK1α, lenalidomide treatment had no significant effect on the degradation of these other CRBN substrates (Fig. 5h). Collectively, these results show that compared to lenalidomide, Wnt signaling activates the function of CRBN towards a wide spectrum of substrates.
CRBN is a positive regulator of Wnt activity
We used a reporter cell line stably expressing a TOPFlash Wnt reporter gene (HEK293STF) in order to begin to probe the role CRBN may play in Wnt pathway activity. We found that CRBN knockdown significantly attenuated Wnt-stimulated reporter gene activity (Fig. 6a and Supplementary Fig. 6a). Additionally, overexpression of CRBN resulted in increased Wnt reporter activity in HEK293STF cells in the absence of exogenous Wnt stimulation (Fig. 6b and Supplementary Fig. 6b). Importantly, this increased Wnt activity was not observed upon ectopic expression of CK1α binding-deficient CRBN mutants (Figs. 4d, 6b and Supplementary Fig. 6b). Furthermore, both Wnt3a and overexpressed CRBN resulted in the degradation of CK1α in these cells (Supplementary Fig. 6a, b). These results are consistent with CRBN regulating Wnt activity in a manner dependent on CK1α.
Mouse intestinal organoids are grown in the presence of exogenous Wnt transform from a budded phenotype to a cystic morphology41 (Fig. 6c, di, ii and Supplementary Fig. 6di). Crbn knockdown significantly attenuated this Wnt-stimulated cystic phenotype, consistent with an inhibition of Wnt activity (Fig. 6diii–iv, e and Supplementary Fig. 6dii-iii). We previously showed that CK1α levels are decreased in intestinal organoids supplemented with exogenous Wnt15. Herein, we find that knockdown of Crbn rescues this Wnt-driven decrease in CK1α levels (Supplementary Fig. 6c). Together, these results show that CRBN is required for Wnt signaling and that this function of CRBN occurs primarily via the regulation of CK1α.
CRBN modulates Wnt function in vivo
Wnt signaling is evolutionarily conserved across phyla, with many pivotal discoveries in the Wnt pathway originating from studies using Drosophila melanogaster42. Wnt/Wingless (Wg) signaling directs cell fate specification during the development of the wing imaginal disc, the precursor of the adult wing43. During this process, the Wg target gene senseless (sens) is transcriptionally activated on either side of a row of Wg-expressing cells at the dorsoventral boundary44 (Fig. 7a, bi–iii and Supplementary Fig. 7a, di–iii). We performed RNAi-mediated knockdown of the CRBN ortholog, ohgata (ohgt)45, in the posterior compartment of the wing imaginal disc and observed significantly disrupted Sens expression across the posterior region of wing disks (Fig. 7biv–vi, c and Supplementary Fig. 7bi–iii, c, div–ix). Similar results were observed upon knockdown of the well-established regulator of Wg signaling disheveled (dsh)46, albeit in a manner that was more penetrant (Supplementary Fig. 7biv–vi, c). Of note, the expression of Wg was not disrupted by ohgt knockdown (Supplementary Fig. 7a, di, ii, iv, v, vii, viii), indicating that Notch signaling, which is required for wg transcription at the dorsoventral margin47, remained intact. These results provide evidence that Ohgt/Crbn promotes Wg/Wnt signal transduction in Drosophila.
Canonical Wnt signaling plays a pivotal role in midline patterning during zebrafish development, with aberrant Wnt activation resulting in eye loss48 and Wnt/β-Catenin inhibition resulting in cyclopia49–52 (Fig. 8a). We injected either crbn mRNA53, crbn morpholino (MO)35, or three distinct guide RNA (sgRNA) sequences targeting crbn along with dCas9 mRNA (CRISPRi), into zebrafish embryos and examined the phenotype of these head structures. Increased expression of crbn mRNA resulted in eye loss (18%) (Fig. 8bii, c) relative to WT zebrafish (Fig. 8bi, c), whereas injection of crbn MO or CRISPRi constructs resulted in increased cyclopia (7% or 15–26%, respectively) (Supplementary Fig. 8ai, iv, b or Fig. 8biii, e, and Supplementary Fig. 8ei, ii) relative to their respective negative controls (Fig. 8bi and Supplementary Fig. 8aiii, b or Fig. 8bi, e, and Supplementary Fig. 8eiii–vi). Consistent with the cyclopic phenotype observed upon crbn knockdown (by MO or CRISPRi) being specific, co-injection with crbn mRNA significantly attenuated this phenotype (Fig. 8biv, g and Supplementary Fig. 8aii, b). Importantly, the crbn knockdown-induced cyclopic phenotype was rescued by co-expression of mRNA encoding β-catenin (Fig. 8bv, g). These results suggest that crbn positively modulates Wnt signaling in zebrafish. Consistently, corresponding changes in the expression of canonical Wnt/β-catenin target genes were observed upon modulation of crbn levels, correlating alterations of crbn levels with the regulation of canonical Wnt signaling in vivo (Fig. 8d, f and Supplementary Fig. 8c, d, f), further supporting the role of zebrafish crbn in canonical Wnt/β-catenin signaling. Moreover, we also noted that Ck1α protein levels in zebrafish were correspondingly regulated by crbn modulation (Supplementary Fig. 9). Together, these results show that CRBN regulates Wnt pathway activity in vivo, which leads to the Wnt-dependent modulation of CK1α abundance and Wnt-relevant phenotypes.
Discussion
These studies show that CRBN is an evolutionarily conserved regulator of the Wnt signaling pathway, whose Wnt-dependent activation results in the ubiquitin-dependent, proteasomal degradation of a subset of substrates. Our results indicate that the regulation of CK1α protein levels is one of the major Wnt-driven functions of CRBN (Fig. 9)21,40. However, the degradation of other Wnt-driven CRBN substrates, such as those identified in our studies, may also represent important regulators of Wnt-driven biology. Our studies highlight an extracellular ligand-stimulated mechanism of regulation for CRBN, via modulation of Wnt activity.
Although we do not fully understand the mechanism by which Wnt regulates CRBN, we show that such regulation relies on the previously described IMiD binding pocket21,40. This finding underscores the significance of this structural motif for endogenous CRBN regulation, which we speculate may function as a binding pocket for an endogenous small-molecule CRBN regulator. Our findings may also have important clinical implications, as CRBN modulators might provide a potential form of pharmacological intervention in Wnt-driven diseases.
Methods
Reagents
Recombinant Wnt3a (R&D Systems) was reconstituted with 0.1% BSA in PBS to 200 ng/μL (human Wnt3a) or to 40 ng/μL (mouse Wnt3a), snap-frozen and stored at −80 °C. Cycloheximide (Sigma), BA (Cell Signaling Technology), MG132 (Selleck Chemicals), lenalidomide (Sigma), the DUB inhibitor PR-619 (Selleck Chemicals), MLN4924 (Selleck Chemicals), and the ROCK inhibitor Y27632 (Stemcell Technologies) were dissolved in 100% DMSO at 3 to 50 mM and stored at −20 °C. Plasmids expressing human Flag-tagged CRBN (NP_001166953.1) and HA-tagged human CK1α (NP_001883.4) were synthesized in pcDNA3.1 (Addgene) and point mutations introduced by Genscript. Smart-pool siRNA targeting specific genes (AXIN1, APC, and CRBN) were purchased (Dharmacon). Individual siRNA targeting CRBN were purchased from Dharmacon (#1: J-021086-09-0005; #2: J-021086-10-0005; #3: J-021086-11-0005). Lentiviral expressing shRNA targeting human CRBN (#1: TL305228V #B; #2: TL205228V #D), mouse Scramble control (TR30021V), or mouse Crbn (#A-D: TL503372V #A, B, C, D) were purchased (Origene).
Cell-based assays
HEK293T (HEK), HEK293STF, NIH3T3, and L-WRN cells were purchased from American Type Culture Collection (ATCC) and maintained as recommended. Cells (50–70% confluent) were treated with recombinant Wnt3a ligands, the indicated drugs or siRNA in media containing 5% FBS. Wnt3a was added at 250 ng/mL to cells for 24 h unless otherwise indicated. About 50 nM of the indicated siRNA was transfected using Lipofectamine RNAiMAX (Thermo Fisher) following the manufacturer’s protocol, for 48 h. Plasmids were transfected using Lipofectamine 2000 (Thermo Fisher) in media containing 10% FBS, for 48 h. Total RNA was extracted from cells using RNeasy plus kit (Qiagen), reverse transcribed using a high-capacity cDNA reverse transcription kit (Applied Biosystems), and used for quantitative PCR using specific TaqMan probes (Invitrogen) (Supplementary Table 1). RNA expression was then analyzed by Bio-Rad CFX manager 3.1 software. For Wnt reporter assays, following treatment HEK293STF cells were lysed in 1X passive lysis buffer (Promega) at room temperature for 10 min, followed by a luciferase assay (Promega). Luminescence was recorded by a Veritas microplate reader. Cytoplasmic and nuclear cell fractions were separated using NE-PER™ Extraction Kit (Thermo Fisher) as per the manufacturer’s protocol.
Immunoblotting and immunoprecipitation
Immunoblotting
Cells were lysed in an SDS sample buffer and boiled for 5 to 10 min, followed by SDS polyacrylamide gel electrophoresis. Proteins were then transferred to a nitrocellulose membrane and immunoblotted. The primary antibodies used were CK1α (1:1000 ab108296) and SIAH1 (1:250, ab2237) from Abcam, Axin1 (1:1000, 2087), APC (1:1000, 2504), GSK3β (1:1000, 9315), phosphorylated β-Catenin S45 (1:1000, 9564), phosphorylated β-Catenin S33, 37, T41 (1:1000, 9561), β-Catenin (1:1000, 9562), c-Myc (1:5000, 5605), GAPDH (1:5000, 8884), c-Jun (1:5000, 9165), PTEN (1:5000, 9552), and α-Tubulin (1:5000, 9099) from Cell Signaling Technology, α-Tubulin (1:5000, T6199) and GS (1:1000, MAB302) from Millipore, Ubiquitin (1:500, sc-8017) and HSP90 (1:5000, sc-13119) from Santa Cruz Biotechnology, P62 (1:1000, 610832) from BD Biosciences, CRBN (1:5000, NBP1-91810) and MEIS2 (1:250, H00004212-M01) from Novus Biologicals, and Flag (1:5000, F7425) from Sigma (Supplementary Table 2). The secondary antibodies used were HRP-conjugated donkey anti-mouse or anti-rabbit (715-035-150 or 711-035-152), or rabbit anti-goat IgG (305-025-045) from Jackson ImmunoResearch (1:10000). ImageJ was used to quantitate the signal of the indicated proteins in immunoblots.
Immunoprecipitation
Cells treated with PBS or Wnt3a for 4 h in the presence of 10 μM MG132 were lysed with Pierce IP lysis buffer (Thermo Fisher) containing a protease and phosphatase inhibitor cocktail (Thermo Fisher) and 10 μM MG132. For immunoprecipitation from HEK cells, the cell extracts were incubated with 1 μg of CRBN antibodies (Abcam ab68763) or PTEN antibodies (Santa Cruz Biotechnology sc-393186) overnight at 4 °C. Protein G agarose beads (Invitrogen) were then added for 1 h at 4 °C. For immunoprecipitation from NIH3T3 cells, CRBN antibodies (Cell Signaling Technology 71810) were first conjugated to agarose beads using an AminoLink Plus Immobilization Kit (Thermo Fisher) and these CRBN-conjugated beads were incubated in cell lysates overnight at 4 °C. Antibody-bound or -conjugated beads were then isolated by centrifugation and washed with lysis buffer for 10 min. For Flag immunoprecipitation, lysates were incubated with Flag antibody-conjugated beads (Sigma) overnight at 4 °C, and the beads were subsequently isolated by centrifugation and washed with lysis buffer for 10 min three times. Antibody-bound or -conjugated beads were resuspended in SDS sample buffer, boiled for 5 min, and used for the subsequent analysis.
Ubiquitination assays
HEK cells were treated with recombinant Wnt3a (100 ng/mL) and MG132 for the indicated amount of time. Cells were lysed with Pierce IP lysis buffer (Thermo Fisher), a protease and phosphatase inhibitor cocktail (Thermo Fisher), 50 μM PR-619, and 10 μM MG132. CK1α antibodies (Abcam ab206652) were conjugated to agarose beads using an AminoLink Plus Immobilization Kit (Thermo Fischer). The beads were incubated in cell lysates overnight at 4 °C, isolated by centrifugation, and washed with lysis buffer. For the tandem ubiquitin-binding entities (TUBE) assay, HEK cells were transfected with a plasmid expressing HA-tagged CK1α and then treated with recombinant Wnt3a (100 ng/ml) and MG132 for 6 h. Cells were then lysed as described above and incubated with TUBE-beads (Boston Biochem) for 2 h at 4 °C. The beads were subsequently isolated by centrifugation and washed with TBS containing 0.1% Tween-20 for 5 min three times. The antibody- or TUBE-conjugated beads were then boiled in an SDS sample buffer for 5 min and analyzed by immunoblotting.
For the in vitro ubiquitination reaction, HEK cells were transfected with a plasmid expressing Flag-CRBN and 48 h later treated with recombinant Wnt3a (100 ng/mL) and MG132 for 4 h. Cells were lysed and Flag-CRBN immunoprecipitated using Flag antibody-conjugated beads (Sigma) as described above. The beads were washed with lysis buffer three times and then once in E3 ligase buffer (Boston Biochem) at 4 °C. The following reagents were added to Flag-CRBN bound beads and 50 ng recombinant GST-CK1α (Invitrogen) in 1X E3 ligase buffer: 200 nM UBE1 (E1), 1 μM UBE2D1 (E2), 1 μM UBE2D3 (E2), 1 μM UBE2G1 (E2), 1 μg K0 ubiquitin, 1 μM ubiquitin aldehyde, 10 μM MG132, and 50 μM PR-619, plus or minus 2.5 mM Mg-ATP (all from Boston Biochem). The reactions were mixed and then incubated at 30 °C for 3 h. The reactions were stopped by boiling with SDS sample buffer for 5 min, followed by immunoblotting.
In vitro binding assay
Flag-CRBN was immunoprecipitated from HEK cells, treated with or without recombinant Wnt3a, using Flag antibody-conjugated beads (Sigma). IgG-conjugated beads (Sigma) served as a negative control for Flag-CRBN isolation. The antibody-conjugated beads were then blocked with 10% BSA in 1 M Tris-HCl (pH 7.25) for 6 h at 4 °C as previously described54. The blocked beads were next washed with 1 M NaCl and subsequently incubated with recombinant GST-CK1α in binding buffer (25 mM HEPES pH 7.25, 100 mM NaCl, 0.01% Triton X-100, 1 mM DTT, and 5% glycerol) for 1 h at 4 °C. After one 5 min wash with binding buffer, the antibody-conjugated beads were resuspended in SDS sample buffer and boiled for 5 min, followed by silver staining (Thermo Scientific) or immunoblotting.
Organoids
Wild-type mouse intestinal organoids were isolated and maintained as previously described15. Prior to shRNA lentiviral infection, organoids were collected and digested by Gentle Cell Dissociation Reagent (Stemcell Technologies) at room temperature for 10 min, followed by washing with basal culture medium. About 10,000 dissociated organoid cells were then resuspended in 25% conditioned L-WRN (Wnt3a, Rspondin3, Noggin) media, produced from L-WRN cells according to ATCC’s protocol, which contained lentivirus particles (MOI = 10), 8 μM polybrene and 10 μM Y27632. Organoid cells were inoculated with the virus using a centrifugation-based protocol: 600 × g for 2 h at room temperature, followed by incubation for 1 h at 37 °C. The cells were again pelleted, washed with culture media, resuspended in Matrigel, plated, and overlaid with basal culture media or 25% L-WRN conditioned media for 5 days. Images were obtained using an Olympus IX51 inverted fluorescence microscope and Olympus CellSens software.
Model organisms
NHGRI-1 zebrafish embryos55 (1 cell) were injected with 15 ng crbn morpholinos (MO) in 3 nL into the yolk or 1 ng crbn mRNA in 1 nL into the single cell. The sequence of crbn mRNA and MO are as previously described35,53. For CRISPR/dCas9 injections, embryos were injected with 250 pg dCas9 mRNA or/and 500 pg crbn sgRNA. For rescue experiments, embryos were injected with 250 pg dCas9 mRNA, 500 pg crbn sgRNA, and 250 pg mRNA encoding Crbn or β-Catenin. Sequences of crbn sgRNA (Alt-R® CRISPR-Cas9 guide RNA, Integrated DNA Technologies) are as following: #A: CACGGCUAUGGCUGCUGAGAGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU; #B:GGAUUGUAAACACACGGCUAGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU; #E: CAGAGCGGAUUGUAAACACAGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU. Embryos were raised, fixed, and phenotyped at 1 dpf or 2 dpf56. Bright field images of embryos were acquired using a Zeiss Stemi 2000-CS microscope with an Olympus DP72 camera. For qPCR analysis, cDNA samples were generated from 20 hpf embryos and cDNA expression analyzed using specific SYBR Green primers (Thermo Fisher) (Supplementary Table 1)57,58, followed by analysis on a Quant Studio 5 real-time PCR system (Applied Biosystems)56. For immunoblotting, 15 hpf embryos were separated into 3–15 embryo pools, homogenized in RIPA buffer (Thermo Fisher) containing a protease and phosphatase inhibitor cocktail (Thermo Fisher) and 10 µM MG132 for 1 h at 4 °C. Lysates were cleared by centrifuging at 8000 × g for 10 min at 4 °C and then boiled in SDS sample buffer for 5 min. After boiling, samples were sonicated for 30 s and used for immunoblotting.
The RNAi lines: ohgti1 (VDRC, #110809), ohgti2 (VDRC #40486)), dshi (Bloomington Drosophila Stock Center, #31306), and yi (VDRC #106068) were expressed in Drosophila third instar male larval wing disks using the hh-Gal4 driver59 in conjunction with UAS-dcr-260 (Bloomington Drosophila Stock Center #25757). All crosses were reared at 25 °C. Drosophila wing disks were then used for immunohistochemistry56. Fluorescent images were obtained with a Nikon A1RSi confocal microscope. To quantitate Sens expression, the length of Sens expressed in the posterior region was measured and normalized to the total length of the posterior compartment.
Animal care
Zebrafish studies were performed following the animal protocol of the University of Maryland’s Institutional Animal Care and Use Committee.
Statistics
A minimum of three independent replicates were performed for each experiment. The error bars shown represent the standard error of the mean (SEM) of at least three independent experiments, except for organoid studies—which instead show the standard deviation (SD) of three technical replicates in one of three independent experiments. Statistical relevance was determined using a two-tailed Student’s t-test, a two-way ANOVA (protein turnover comparisons), or a Fisher’s exact test (Drosophila and zebrafish phenotype studies) using Prism Graphpad 9. Asterisks indicate statistical significance (*p values ≤ 0.05; **p values ≤ 0.01; ***p values ≤ 0.001; ****p values ≤ 0.0001).
Reporting Summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Supplementary information
Acknowledgements
We thank all members of the Robbins, Capobianco, Lee, and Ahmed laboratories for their insightful advice and discussion regarding this work. We also thank GUMC Computational Chemistry Shared Resources (CCSR) for structural insights in this work. This work was supported by the National Institutes of Health (NIH) Grants: R01CA219189 to D.J.R., R35GM122516 to E.L., R01GM121421, R01GM122222, and R35GM136233 to Y.A., R01CA244188 to D.J.R., E.L., and Y.A., and R01GM118557 to C.C.H. This work was also supported by the Dwoskin Cancer Fund and the National Cancer Institute of the NIH under Award Number P30CA240139 to Sylvester Comprehensive Cancer Center.
Source data
Author contributions
C.S. and D.J.R. designed this project. E.L., Y.A., and N.G.A. provided insights with project design. C.S., A.N., L.R.N., A.A.A., M.S.-I., L.M.S., H.B., H.W., N.B., B.L., and S.D. obtained data and provided experimental support. C.S., A.N., L.R.N., A.A.A., L.M.S., H.B., N.B., S.D., and C.H.W. analyzed and interpreted the data. C.S. and D.J.R. wrote the manuscript. E.L., Y.A., N.G.A., B.L., H.B., N.B., C.C.H., and A.J.C. critically revised the manuscript. A.N., D.T.W., F.Y., and M.G.-C. proofread the manuscript.
Data availability
All data supporting the findings of this study are available in the paper and the Supplementary information file. Raw data and original gel images are included in the Source Data file. All other relevant data were available from the authors upon reasonable request. Source data are provided with this paper.
Competing interests
D.J.R., E.L., and A.J.C. are founders of StemSynergy Therapeutics Inc., a company commercializing small-molecule cell signaling inhibitors, including Wnt inhibitors. The remaining authors declare no competing interests.
Footnotes
Peer review informationNature Communications thanks the anonymous reviewers for their contributions to the peer review of this work. Peer review reports are available.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
These authors contributed equally: Chen Shen, Anmada Nayak.
Supplementary information
The online version contains supplementary material available at 10.1038/s41467-021-25634-z.
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Data Availability Statement
All data supporting the findings of this study are available in the paper and the Supplementary information file. Raw data and original gel images are included in the Source Data file. All other relevant data were available from the authors upon reasonable request. Source data are provided with this paper.