In the article entitled “Protective effect of hydrogen sulfide on endothelial cells through Sirt1-FoxO1-mediated autophagy” (1), the images of Figure 5B selected to represent the flow cytometric analysis of the apoptosis of HUVECs in Ox-LDL group and Ex-527 + GYY4137 + Ox-LDL group were duplicated incorrectly.
Figure 5.
H2S-induced autophagy via Sirt1 protects against Ox-LDL-induced apoptosis in HUVECs. HUVECs were pre-treated with or without 3-MA, resveratrol, Ex-527, and GYY4137 for the indicated times followed by treatment with Ox-LDL. (A) Immunoblot analyses showing cleaved-caspase-3 and cleaved-PARP. Expression in control (Ctr) group cells was assigned a value of 1, n≥3. **P<0.01 versus Ctr, #P<0.05 versus Ox-LDL. (B,C) Flow cytometric analysis to detect the apoptosis of HUVECs. n≥3. **P<0.01 versus Ctr, #P<0.05 versus Ox-LDL. (D) Cell viability was measured using the CCK-8 assay. Cell viability in control (Ctr) group was assigned a value of 1, n=6. *P<0.01 versus Ctr, #P<0.05 versus Ox-LDL. Data are expressed as the mean ± SEM. (E) Schematic representation of the effects and mechanisms of H2S on autophagy and apoptosis in HUVECs.
The revised version of Figure 5, containing the correct data for the Ex-527 + GYY4137 + Ox-LDL in Figure 5B, is shown below. This error did not significantly affect either the results or the conclusions of the paper.
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References
- 1.Zhu L, Duan W, Wu G, et al. Protective effect of hydrogen sulfide on endothelial cells through Sirt1-FoxO1-mediated autophagy. Ann Transl Med 2020;8:1586. 10.21037/atm-20-3647 [DOI] [PMC free article] [PubMed] [Google Scholar]