For Fig. 3A, the schematic representation of the structure of Man7D3 was mistakenly duplicated with the one of Man5 during article preparation. The corrected images are presented in the revised Fig. 3A. In Table 1, there is an error in one of the exponential values. The error is only in the exponential term of Ka value and there is no change in Kd. This correction does not affect the results or the conclusions of the work. The authors apologize for inconvenience these errors may have caused readers.
Table 1. Thermodynamic parameters of manno-oligosaccharide binding to horcolin at 25 °C
| Ligand | N | KA (M-1) | KD | ΔH (kcal/mol) | ΔG (kcal/mol) | TΔS (kcal/mol) |
|---|---|---|---|---|---|---|
| Me-α-manno pyranoside | 1.72 ± 0.328 | 200 ± 27.8 | 5.00 mM | –6.324 | –3.1354 | –3.1886 |
| α-D-Man | 1.90 ± 0.521 | 101 ± 12.5 | 9.90 mM | –5.517 | –2.73368 | –2.78332 |
| Manα1-2-Man | 1.946 ± 0.4028 | 398.9 ± 41.42 | 2.50 mM | –8.974 | –3.5504 | –5.4236 |
| Manα-1-3-Man | 1.88 ± 0.0724 | 596 ± 14.6 | 1.67 mM | –10.46 | –3.7848 | –6.6752 |
| Manα-1-6-Man | 1.78 ± 0.243 | 227 ± 10.3 | 4.40 mM | –14.88 | –3.2282 | –11.6518 |
| Manα-1-4-Man | 1.77 ± 0.649 | 196 ± 21.2 | 5.10 mM | –10.28 | –3.128 | –7.152 |
| Man3 | 2.01 ± 0.0275 | 6.10 E3 ± 300 | 163 μM | –12.94 | –5.1622 | –7.7778 |
| Man5 | 0.774 ± 0.076 | 5.99 E4 ± 1.00 E5 | 16.69 μM | –16.76 | –6.5088 | –10.2512 |
| Man7-D1 | 0.925 ± 0.0117 | 2.61 E5 ± 2.87 E4 | 3.831 μM | –17.1 | –7.3256 | –9.7744 |
| Man7-D3 | 1.09 ± 0.00711 | 2.4 E5 ± 1.45 E4 | 4.16 μM | –15.99 | –7.348 | –8.642 |
| Man9 | 1.34 ± 0.018 | 8.60 E4 ± 1.21 E4 | 11.6 μM | –8.277 | –6.73038 | –1.54662 |
Abbreviations: N, stoichiometry coefficient (number of sugars bound per mole of horcolin monomer); ΔH, enthalpy; ΔS, entropy; ΔG, reaction energy (calculated with the formula ΔG = ‒RT ln Kb, where R = 1.987 cal/mol_K).
VOLUME 295 (2020), PAGES 12111–12129