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. 2021 Aug 23;10:e66738. doi: 10.7554/eLife.66738

Figure 2. White matter microglia (WM) repopulation elicits a dynamic wave of repopulating proliferative myeloid cells.

(A) Spatial heat map of WM repopulation at 7 day recovery in three brain positions (Bregma 2.58 mm, 1.10 mm, −2.06 mm) depicting % of brains with cells present in specific brain regions (n=5–19). (B–C) Immunofluorescence whole brain coronal (B) and sagittal (C) section images of IBA1+ cells (green) show a sequential time course of the ‘wave’ (see higher resolution image of wavefront in B1 insert) of myeloid cells filling the brain between 7 and 14 day recovery during WM repopulation. White dotted lines and yellow arrows indicate the edge and direction of the ‘wave’, highlighting the radial migratory patterns of WM repopulating cells. Yellow dashed arrows indicate the direction of the tangential migratory pattern of WM repopulating cells, mostly utilizing WM tracts. The straight white dashed line in C shows the Bregma position at which coronal sections were taken for B. (E–F) Representative immunofluorescence whole brain images of myeloid cells (IBA1, green) and proliferating cells (Ki67, red) at 7 (A) and 14 (B) day recovery. Insets show higher resolution of IBA1 and Ki67 colocalization in initially repopulating cells (A1) and in cells outside and inside the wavefront (B2-4). Data are represented as mean ± SEM (n=3–8).

Figure 2—source data 1. White matter microglia (WM) repopulation elicits a dynamic wave of repopulating proliferative myeloid cells.

Figure 2.

Figure 2—figure supplement 1. White matter microglia (WM) repopulation elicits a dynamic wave of repopulating proliferative myeloid cells.

Figure 2—figure supplement 1.

(A) Immunofluorescence whole brain coronal section images of IBA1+ cells (green) show the Stages (i.e. sequential time course), including the symmetrical distribution and expansion, of the ‘wave’ of repopulating myeloid cells that fill the brain during WM repopulation. White lines separate different brain regions: corpus callosum (CC), cortex (CTX), caudoputamen (CP), and lateral ventricle (LV). White dotted lines and yellow arrows indicate the edge and direction of the ‘wave’, highlighting the radial migratory patterns of WM repopulating cells. Yellow dashed arrows indicate the direction of the tangential migratory pattern of WM repopulating cells, mostly utilizing WM tracts. (B) Representative tile scan confocal images of the migration of WM repopulating IBA1+ cells (green) out of the WM tracts and into cortical regions. White dotted lines indicate WM boundaries.