Table 3.
Activity level and movement speed of wild-type flies, flies with suppressed EIP-ring or P-ring neurons, and flies with photoactivated EIP-ring or P-ring neurons
| Wild-type and neural deficits |
Genotype | Activity level (%) | Speed (mm/s) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Prestimulus stage |
Stimulus stage |
Poststimulus stage |
Prestimulus stage |
Stimulus stage |
Poststimulus stage |
||||||
| Wild type | w+ | 69.78 ± 4.59 |
69.35 ± 3.32 |
64.52 ± 3.96 |
13.18 ± 0.91 |
12.18 ± 0.70 |
13.22 ± 0.77 |
||||
| Suppression of EIP-ring neurons |
32°C, c105-GAL4, tub-GAL80ts;;UAS-Kir2.1 | 81.38 ± 0.57 |
83.39 ± 1.05 |
83.91 ± 0.69 |
7.90 ± 0.25 |
8.89 ± 0.60 |
9.01 ± 0.44 |
||||
| 32°C, c105-GAL4 tub-GAL80ts;;UAS-TNT | 82.30 ± 0.78 |
83.80 ± 0.66 |
81.49 ± 0.64 |
8.57 ± 0.41 |
9.46 ± 0.29 |
8.43 ± 0.38 |
|||||
| Suppression of P-ring eurons |
32°C, ;;VT5404-GAL4, tub-GAL80ts/ UAS-Kir2.1 | 83.33 ± 1.10 |
84.62 ± 1.44 |
82.99 ± 1.53 |
8.44 ± 0.59 |
9.30 ± 0.84 |
9.19 ± 0.92 |
||||
| 32°C, ;;VT5404-GAL4, tub-GAL80ts/ UAS-TNT | 86.44 ± 1.56 |
87.51 ± 1.19 |
85.53 ± 1.04 |
11.26 ± 1.37 |
11.91 ± 1.20 |
10.70 ± 0.80 |
|||||
| Neural deficits | Genotype | Activity level (%) | Speed (mm/s) | ||||||||
| Prestimulus stage |
Stimulus stage first 30 s |
Stimulus stage last 30 s |
Poststimulus stage |
Prestimulus stage |
Stimulus stage first 30 s |
Stimulus stage last 30 s |
Poststimulus stage |
||||
| Photoactivation of EIP-ring neurons |
c105-GAL4;; UAS-CsChrimson.mVenus | 71.57 ± 3.79 |
66.20 ± 5.39 |
28.81 ± 4.26 |
55.84 ± 3.76 |
12.86 ± 0.81 |
10.96 ± 0.73 |
5.80 ± 0.57 |
9.19 ± 0.80 |
||
| Photoactivation of P-ring neurons |
;;VT5404-GAL4/UAS-
CsChrimson.mVenus |
84.65 ± 6.20 |
85.71 ± 3.79 |
72.40 ± 7.56 |
68.42 ± 7.92 |
15.26 ± 1.01 |
13.27 ± 0.92 |
11.88 ± 1.15 |
13.58 ± 0.82 |
||
| Neural deficits | Genotype | Activity level (%) | Speed (mm/s) | ||||||||
| Prestimulus stage |
Stimulus stage |
Poststimulus stage 0–20 s |
Poststimulus stage 20–30 s |
Poststimulus stage 30–90 s |
Prestimulus stage |
Stimulus stage |
Poststimulus stage 0–20 s |
Poststimulus stage 20–30 s |
Poststimulus stage 30–90 s |
||
| Photoactivation of EIP-ring neurons |
c105-GAL4;; UAS-
CsChrimson.mVenus |
68.38 ± 4.19 |
70.79 ± 4.13 |
69.87 ± 5.50 |
34.25 ± 6.84 |
58.84 ± 4.15 |
10.41 ± 0.55 |
10.06 ± 0.65 |
8.95 ± 0.87 |
5.98 ± 0.67 |
8.70 ± 0.57 |
| Photoactivation of P-ring neurons |
;;VT5404-GAL4/UAS-
CsChrimson.mVenus |
81.32 ± 3.91 |
77.41 ± 3,42 |
75.17 ± 4.95 |
79.71 ± 3.02 |
76.51 ± 3.07 |
14.68 ± 0.78 |
14.29 ± 0.61 |
13.99 ± 0.87 |
12.05 ± 0.90 |
13.27 ± 0.58 |
| Wild type | Genotype | Activity leve (%) |
Speed (mm/s) |
||||||||
| Amputating the unilateral foreleg |
w+ | 71.04 ± 6.66 |
8.50 ± 0.75 |
||||||||