Figure 7.

Innexin2 knockdown affects the amplitude of PDF oscillation in the s-LNv dorsal projection

(A) Scatterplots of PDF intensity in the s-LNv dorsal projection of both the control (dcr; Inx2 RNAi) and experimental (Clk856 > dcr; Inx2 RNAi) flies plotted at different time-points over a 24-hr cycle on the third day of DD. Each dot represents the mean PDF intensity value averaged over both the hemispheres of one brain. The black and red lines are the best-fit COSINE curve from the parameters that were extracted from the COSINOR analysis.

(B) Polar-plots depicting the acrophase of PDF oscillation in control (dcr; Inx2 RNAi) and experimental flies (Clk856 > dcr; Inx2 RNAi). The phase values obtained after COSINOR curve fitting are shown as solid lines and the error values (95% CI) are depicted as dashed lines around the mean. Overlapping error bands indicate that the experimental phases are not significantly different from controls.

(C) Amplitude values obtained from COSINOR curve fits are plotted for control and experimental flies. Error bars are 95% CI values calculated from the standard error obtained from COSINOR analysis. Non-overlapping error bars indicate that experimental lines are significantly different from controls. COSINOR analysis was implemented using the CATCosinor function from the CATkit package written for R (Lee Gierke and Cornelissen, 2016) See Table 3; Figure S10 for more details. n > 9 brain samples both in case of control and experimental flies for all timepoints.