Skip to main content
. 2021 Jul 23;40(36):5548–5558. doi: 10.1038/s41388-021-01956-6

Fig. 5. LPAR1 plays an important role in pulmonary metastasis of osteosarcoma.

Fig. 5

A Luminescence of Akaluc-transduced MG-63 cells. MG-63/Akaluc/sgCTRL and MG-63/Akaluc/sgLPAR1#1 cells were seeded at the indicated cell number and treated with 50 μM AkaLumine-HCl. Luminescence was measured with the Mithras LB940 Multimode Microplate Reader. B Cell proliferation of LPAR1 KO MG-63 cells. MG-63/Akaluc/sgCTRL and MG-63/Akaluc/sgLPAR1#1 cells were seeded and assessed by CellTiter-Glo Reagent at the indicated time points. All data are shown as means ± SD (n = 4). n.s. as determined by Student’s t test. C, D BLIs of mice intravenously injected Akaluc-transduced MG-63 cells. 1 × 106 cells of MG-63/Akaluc/sgCTRL or MG-63/Akaluc/sgLPAR1#1 were intravenously injected into SCID-beige mice. Mice were intraperitoneally injected with 100 μL of 5 mM AkaLumine-HCL and underwent BLI. Images were taken 3 h and 7 days after cell injection (C), and their total flux in lung was calculated (C). **p < 0.01 as determined by the Mann–Whitney U test.