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. 2021 Sep 10;9:185. doi: 10.1186/s40168-021-01126-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — EPA and DHA promote SCFA production and beiging of WAT (n = 6 male and n = 6 female per group). a Individual SCFA levels in cecum content. b, c GLP-1 concentrations in small intestinal. d Serum LPS levels. e mRNA expression of TLR4, GPR41, GPR43, IL6, and CCL2 in WAT. f Representative images of UCP1 and CD137 staining in adipose tissues. Scale bar, 100 μm. g Representative adipose immunoblots for UCP1, CD137, PRDM16, and PPARγ. h Representative adipose immunoblots for GLUT4, p-AKT, and t-AKT. Graph bars with different superscript letters are significantly different (P < 0.05)