Abstract
The most affected disease in recent years is Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2) that is notable as COVID-19. It has been started as a disease in one place and arisen as a pandemic throughout the world. A serious health problem is developed in the lungs due to the effect of this coronavirus. Sometimes it may result in death as a consequence of extensive alveolar damage and progressive respiratory failure. Hence, early detection and appropriate diagnosis of corona virus in patient’s body is very essential to save the lives of affected patients This work evolves a Silicon (Si) based label-free electrical device i.e. the reduced graphene oxide field-effect transistor (rGO FET) for SARS-CoV-2 detection. Firstly rGO FET functionalized with SARS-CoV-2 monoclonal antibodies (mAbs). Then the rGO FET characteristic response is observed to detect the antibody-antigen reaction of SARS-CoV-2 with different molar ranges. The developed GFET shows better performance towards the drain current and limit-of-detection (LoD) up to 2E-18 M. Therefore, we believe that an intense response was observed than the earlier developed devices and signifies impressive capability for subsequent implementation in point-of-care (PoC) diagnostic tests.
Keywords: FET, SARS-CoV-2, rGO, Biosensor, Limit of detection
Acknowledgements
The authors would like to acknowledge Indian Institute of Technology Hyderabad (IIT Hyderabad) for backing us with some experimental work carried out and the tool (COMSOL Semiconductor Module) required for simulating this work. And M. Durga Prakash thankfully acknowledges this publication as an outcome of the R&D work undertaken project under the Start-up Research Grant (File No.: SRG/2019/002236) scheme of Department of Science and Technology (DST), Government of India, being Science Engineering Research Broad (SERB).
Author Contributions
M. Durga Prakash, B. Vamsi Krsihna and Shaik Ahmadsaidulu: Conceptualization; M. Durga Prakash, B. Vamsi Krsihna and Shaik Ahmadsaidulu: investigation; M. Durga Prakash, Shaik Ahmadsaidulu, B. Vamsi Krsihna, and B: resources; M. Durga Prakash, Shaik Ahmadsaidulu, B. Vamsi Krsihna and Surapaneni Sai Tarun Teja, D Jayanthi, Alluri Navaneetha and P Rahul Reddy: data curation; M. Durga Prakash, Surapaneni Sai Tarun Teja, D Jayanthi, Alluri Navaneetha, P Rahul Reddy and Shaik Ahmadsaidulu: writing—original draft preparation; M. Durga Prakash, B. Vamsi Krsihna, and Shaik Ahmadsaidulu: writing—review and editing; M. Durga Prakash and Shaik Ahmadsaidulu: visualization; M. Durga Prakash: supervision;
Data Availability
No supplementary materials.
Declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Human and Animal Rights
This article does not contain any studies with human or animal subjects.
Consent to Participate
Additional informed consent was obtained from M. Durga Prakash identifying information is included in this article.
Footnotes
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