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. 2021 Aug 19;10:e70272. doi: 10.7554/eLife.70272

Figure 9. Absence of TbFUT1 disturbs mitochondrial activity.

Bloodstream form (BSF) wild-type and TbFUT1 cKO parasites were cultured for 5 days under permissive (+Tet) and non-permissive (-Tet) conditions, fixed and labelled with MitoTracker for mitochondrial potential and with anti-mitochondrial ATPase antibody. In mutants grown in non-permissive conditions (lower panels), both ATPase and MitoTracker staining are strongly reduced, suggesting reduced mitochondrial functionality. Scale bar: 3 μm.

Figure 9.

Figure 9—figure supplement 1. Absence of TbFUT1 disturbs mitochondrial activity.

Figure 9—figure supplement 1.

Still viable bloodstream form conditional null mutants cultured for 5 days under non-permissive conditions (-Tet) were tested with MitoTracker for mitochondrial activity and co-stained with either DAPI (60× objective) (A) or with DAPI and anti-ATPase antibody (B) (20× objective). In both cases, a strong reduction in MitoTracker staining was observed by indirect immunofluorescence. Scale bars: 10 μm.