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. 2021 Aug 27;24(9):103038. doi: 10.1016/j.isci.2021.103038

Figure 8.

Figure 8

Analysis of key proteins related to mitochondrial biogenesis after radiation

(A) Gate strategy for flow cytometric analysis.

(B) Mean fluorescence intensity (MFI) of COX I obtained by flow cytometric analysis at 6 h and 12 h, respectively.

(C) Mean fluorescence intensity of SDHA obtained by flow cytometric analysis at 6 h and 12 h, respectively.

(D) Effect of irradiation on the activity of citrate synthase (CS). HeLa cells were exposed to gamma-ray at doses of 1 Gy, 2 Gy and 4 Gy, respectively. After 6, 24 hours, the expression level of citrate synthase activity was detected by UV-Vis spectrophotometer.

(E) Western blot assay of the related mitochondria biogenesis proteins after radiation treatment. HeLa cells were treated with different doses of irradiation (0, 1, 2, 4 Gy), and total proteins were extracted at different time points. The WB assay shows the changes of expression of mitochondrial biogenesis marker proteins (PGC-1α, p-AMPK, NRF1, TFAM, Hsp60, and Mfn1) with time.