Video 2. Wild-type vs. mutant melanin patterns.

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This video shows a comparison between a representative wild-type 5 days post-fertilization (dpf) zebrafish larva and two representative pigmentation mutant 5 dpf larvae which have been stained with silver and X-ray microtomography (micro-CT) imaged. Visualization settings have been kept constant to facilitate direct comparisons between normalized samples. In volume renderings, the golden mutant (slc24a5^b1/b1) shows markedly reduced melanin content throughout the body and eyes. The nacre mutant (mitfa^w2/w2, head scan rendering only) shows essentially no body pigment but near-normal retinal pigment epithelia (RPE). Finally, stained volumes segmented by anatomic regions show the golden mutant exhibiting wild-type overall pigment organization while the nacre mutant (head scan rendering only) lacks the body stripes. Background music by DJ Vanselow.