Table 2. Statistical analysis of wild-type, slc24a5b1/b1, and mitfaw2/w2 samples for segmented pigment regions.
| Pigment region | Segmented volume statistics | |
|---|---|---|
| One-way ANOVA F-statistic | p-Value | |
| Total | F(2,6) = 178.43 | 0.000005 |
| RPE (right) | F(2,6) = 36.37 | 0.000442 |
| RPE (left) | F(2,6) = 21.77 | 0.002 |
| RPE (total) | F(2,6) = 29.64 | 0.001 |
| Dorsal stripe | F(1,4) = 145.98 | 0.000269 |
| Ventral stripe | F(1,4) = 376.02 | 0.000042 |
| Yolk sac stripe | F(1,4) = 22.04 | 0.009 |
| Lateral stripe (right) | F(1,4) = 39.01 | 0.003 |
| Lateral stripe (left) | F(1,4) = 17.48 | 0.014 |
| Other | F(2,6) = 52.86 | 0.000155 |
| Body (total) | F(2,6) = 939.89 | 0.000000032209 |
| Pigment region | Cumulative sum of intensity statistics | |
| One-way ANOVA F-statistic | p-Value | |
| Total | F(2,6) = 185.76 | 0.000004 |
| RPE (right) | F(2,6) = 54.69 | 0.000141 |
| RPE (left) | F(2,6) = 70.40 | 0.000068 |
| RPE (total) | F(2,6) = 70.50 | 0.000068 |
| Dorsal stripe | F(1,4) = 138.02 | 0.000300 |
| Ventral stripe | F(1,4) = 1770.94 | 0.000002 |
| Yolk sac stripe | F(1,4) = 35.30 | 0.004 |
| Lateral stripe (right) | F(1,4) = 56.79 | 0.002 |
| Lateral stripe (left) | F(1,4) = 17.62 | 0.014 |
| Other | F(2,6) = 166.28 | 0.000006 |
| Body (total) | F(2,6) = 3690.79 | 0.00000000049447 |
F-statistics for each comparison are reported as F(degrees of freedom between groups, degrees of freedom within groups)= F-statistic. p < 0.05 indicates a significant difference between groups. While the melanin stripes were compared between the wild-type and golden samples, total melanin, RPE melanin, and other melanin were compared between all three genotypes. For these regions, Tukey post hoc tests were used to determine which groups differed significantly from wild type.