Table 4. Quantification of volume and cumulative sum of intensity for mitfa^w2/w2 samples for segmented pigment regions.

mitfaw2/w2 specimen	#1§	#2§	#3§	Average	SD	% Change from wt	p-Value
Pigment region	Segmented volume (µm3)
Total	1.64 × 106	2.03 × 106	1.72 × 106	1.80 × 106	± 2.04 x 105	–63.1	0.000015*
RPE (right)	7.99 × 105	1.00 × 106	7.93 × 105	8.64 × 105	± 1.18 x 105	–26.0	0.032*
RPE (left)	8.41 × 105	1.02 × 106	9.21 × 105	9.28 × 105	± 9.09 x 104	–24.2	0.120*
Other	4.22 × 103	5.02 × 103	5.29 × 103	4.84 × 103	± 5.57 x 102	–92.5	0.000169*
Pigment region	Cumulative sum of intensity (normalized units)
Total	1.97 × 1011	2.64 × 1011	1.91 × 1011	2.17 × 1011	± 4.05 x 1010	–55.0	0.000040*
RPE (right)	1.01 × 1011	1.33 × 1011	8.80 × 1010	1.07 × 1011	± 2.30 x 1010	–25.4	0.040*
RPE (left)	9.54 × 1010	1.31 × 1011	1.03 × 1011	1.10 × 1011	± 1.86 x 1010	–31.2	0.010*
Other	2.40 × 108	2.70 × 108	2.77 × 108	2.62 × 108	± 1.98 x 107	–95.0	0.000007*

*p-Values determined by Tukey post hoc test following one-way ANOVA. § = head segment only analyzed. p-Values were considered significant <0.05. Raw data provided in Table 4—source data 1.