Figure 3. Adar^{P195A/p150−} mice develop organ-specific pathology.

(A) Representative histology of kidney and liver from Adar^{P195A/p150−} mice measured at 23 days of age.

(B) Representative histology of kidney and liver from Adar^{P195A/p150−}Ifih1^−/− mice measured at 23 days of age.

(C) Representative histology of kidney, liver, and spleen from Adar^{P195A/p150−} mice measured at 147 days of age. Arrows indicate glomeruli in kidneys, asterisk indicates region of cytoplasmic vacuolation in liver with small arrow showing microvesicular vacuolation and large arrow showing macrovesicular vacuolation.

(D-F) Histological scores in the kidney, liver, and spleen, measured at 23 days of age. Adar p150^+/+ (n=5); Adar^P195A/p150+ (n=5); Adar^{P195A/p150−} (n=6); Adar^P195A/p150+Ifih1^−/− (n=4); Adar^P195A/p150Ifih1^−/− (n=5).

(G) Representative (of 3 of each genotype) immunofluorescence of the kidney for Adar^{P195A/p150−} and Adar^P195/p150+ mice. DAPI is blue. IgG is red.

(H) Ifnb transcript measured by qRT-PCR in BMMs of the indicated genotypes, with and without 24 hours of treatment with recombinant mouse IFNβ. Adar^P195A/p150+ (n=4); Adar^{P195A/p150−} (n=4); Adar^P195A/p150+Ifih1^−/− (n=3); Adar^{P195A/p150−}Ifihi1^−/− (n=5).