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. 2021 Sep 15;8(6):e1068. doi: 10.1212/NXI.0000000000001068

Retinal Optical Coherence Tomography in Neuromyelitis Optica

Frederike Cosima Oertel 1, Svenja Specovius 1, Hanna G Zimmermann 1, Claudia Chien 1, Seyedamirhosein Motamedi 1, Charlotte Bereuter 1, Lawrence Cook 1, Marco Aurélio Lana Peixoto 1, Mariana Andrade Fontanelle 1, Ho Jin Kim 1, Jae-Won Hyun 1, Jacqueline Palace 1, Adriana Roca-Fernandez 1, Maria Isabel Leite 1, Srilakshmi Sharma 1, Fereshteh Ashtari 1, Rahele Kafieh 1, Alireza Dehghani 1, Mohsen Pourazizi 1, Lekha Pandit 1, Anitha D'Cunha 1, Orhan Aktas 1, Marius Ringelstein 1, Philipp Albrecht 1, Eugene May 1, Caryl Tongco 1, Letizia Leocani 1, Marco Pisa 1, Marta Radaelli 1, Elena H Martinez-Lapiscina 1, Hadas Stiebel-Kalish 1, Sasitorn Siritho 1, Jérome de Seze 1, Thomas Senger 1, Joachim Havla 1, Romain Marignier 1, Alvaro Cobo Calvo 1, Denis Bichuetti 1, Ivan Maynart Tavares 1, Nasrin Asgari 1, Kerstin Soelberg 1, Ayse Altintas 1, Rengin Yildirim 1, Uygur Tanriverdi 1, Anu Jacob 1, Saif Huda 1, Zoe Rimler 1, Allyson Reid 1, Yang Mao-Draayer 1, Ibis Soto de Castillo 1, Axel Petzold 1, Ari J Green 1, Michael R Yeaman 1, Terry Smith 1, Alexander U Brandt 1,*, Friedemann Paul 1,*,
PMCID: PMC8448522  PMID: 34526385

Abstract

Background and Objectives

To determine optic nerve and retinal damage in aquaporin-4 antibody (AQP4-IgG)-seropositive neuromyelitis optica spectrum disorders (NMOSD) in a large international cohort after previous studies have been limited by small and heterogeneous cohorts.

Methods

The cross-sectional Collaborative Retrospective Study on retinal optical coherence tomography (OCT) in neuromyelitis optica collected retrospective data from 22 centers. Of 653 screened participants, we included 283 AQP4-IgG–seropositive patients with NMOSD and 72 healthy controls (HCs). Participants underwent OCT with central reading including quality control and intraretinal segmentation. The primary outcome was thickness of combined ganglion cell and inner plexiform (GCIP) layer; secondary outcomes were thickness of peripapillary retinal nerve fiber layer (pRNFL) and visual acuity (VA).

Results

Eyes with ON (NMOSD-ON, N = 260) or without ON (NMOSD-NON, N = 241) were assessed compared with HCs (N = 136). In NMOSD-ON, GCIP layer (57.4 ± 12.2 μm) was reduced compared with HC (GCIP layer: 81.4 ± 5.7 μm, p < 0.001). GCIP layer loss (−22.7 μm) after the first ON was higher than after the next (−3.5 μm) and subsequent episodes. pRNFL observations were similar. NMOSD-NON exhibited reduced GCIP layer but not pRNFL compared with HC. VA was greatly reduced in NMOSD-ON compared with HC eyes, but did not differ between NMOSD-NON and HC.

Discussion

Our results emphasize that attack prevention is key to avoid severe neuroaxonal damage and vision loss caused by ON in NMOSD. Therapies ameliorating attack-related damage, especially during a first attack, are an unmet clinical need. Mild signs of neuroaxonal changes without apparent vision loss in ON-unaffected eyes might be solely due to contralateral ON attacks and do not suggest clinically relevant progression but need further investigation.


Patients with neuromyelitis optica spectrum disorder (NMOSD) experience recurrent optic neuritis (ON),1 resulting in vision loss and decreased quality of life.1-4 According to our understanding, there are at least 3 subtypes based on serostatus: Up to 3 of 4 patients manifest as anti–aquaporin-4 IgG (AQP4-IgG) seropositive. Approximately half of the AQP4-IgG–seronegative patients manifest as anti–myelin oligodendrocyte glycoprotein IgG (MOG-IgG) seropositive, and half are double seronegative.5 Yet, clinical correlates of serologic phenotypes, including subclinical or clinical retinal degeneration and vision loss, remain unclear.6,7

Optical coherence tomography (OCT) is an interferometric technique producing high-resolution retinal images.1,8 OCT has become a reliable tool for diagnosing and monitoring neurologic and neuro-ophthalmologic diseases, especially for quantifying neurodegeneration after ON.1 Because of limited samples and varying methods, existing OCT studies in NMOSD are inconsistent regarding the amount of retinal neurodegeneration with and without ON. Previous studies also failed to address the influence of retinal neurodegeneration on microcystic macular edema (MME) and function.2,6,7 These issues together with heterogeneities and the often monocentric character of previous cohorts limit the relevance of meta-analyses.

To overcome these limitations, we performed an OCT analysis of AQP4-IgG–seropositive patients with NMOSD in an international multicenter study, termed Collaborative Retrospective Study on retinal OCT in Neuromyelitis Optica (CROCTINO).9 It represents the largest NMOSD OCT data set and additionally validated an OCT postprocessing approach to circumvent differences in acquisition and imaging processing protocols inherent to pooled analyses.10 Outcomes include: (1) distinguishing retinal neurodegeneration after ON from subtle damage in clinically unaffected eyes, (2) defining frequency of MME, and (3) deriving structure-function correlations.

Methods

Study Design

This cross-sectional international multicenter study was performed under the aegis of the CROCTINO study, which was a collaborative effort within the Guthy-Jackson Charitable Foundation network.9 Participating centers contributed OCT data (acquired between 2008 and 2018) and clinical metadata (acquired between 2000 and 2018, eTable 1, links.lww.com/NXI/A557).

Cohort Selection

Inclusion criteria for this analysis were (1) patients diagnosed with NMOSD per the 2015 International Panel of NMOSD diagnosis criteria11 and (2) having confirmed serum AQP4-IgG. Exclusion criteria were (1) comorbidities potentially confounding interpretation of OCT results (e.g., macular degeneration, glaucoma, and intracranial hypertension); (2) > 3 months distance between clinical and OCT data acquisition; (3) < 6 months between OCT imaging and most recent ON, or (4) an uncertain history of ON. The inclusion and exclusion criteria are depicted in eFigure 1, links.lww.com/NXI/A556. AQP4-IgG testing was performed at the discretion of each investigator.

Standard Protocol Approvals, Registrations, and Patient Consents

All participants gave written informed consent, and the study was approved by local ethics committees and conducted in accordance with the applicable laws and the current version of the Declaration of Helsinki. Data are reported according to STROBE reporting guidelines.12

OCT

High-resolution imaging data were acquired using 3 different spectral domain OCT devices: Spectralis SD-OCT (Heidelberg Engineering, Heidelberg, Germany) at 15 centers (194 patients/358 eyes; 72 healthy controls [HCs]/136 eyes); Cirrus HD-OCT (Carl Zeiss Meditec, Dublin, CA) at 6 centers (58 patients/87 eyes); or Topcon 3D OCT-1 (Topcon, Tokyo, Japan) at 1 center (31 patients/56 eyes). All reading of OCT data was performed at Charité–Universitätsmedizin Berlin Translational Neuroimaging Group by 5 graders. Image quality was assessed using modified OSCAR-IB criteria by one of the graders, respectively.13,14 OCT segmentation for the combined ganglion cell and inner plexiform (GCIP) layer and inner nuclear layer (INL) thicknesses was corrected semi-automatically using an in-house software.10,15 In brief, GCIP layer and INL thicknesses were calculated from a 5-mm diameter cylinder around the fovea excluding the central 1-mm diameter cylinder from a macular volume scan.10 The peripapillary retinal nerve fiber layer (pRNFL) thickness was measured and corrected according to the device protocol (Spectralis: peripapillary ring scan with 12° or approximately 3.4 mm diameter around the optic disc; Topcon and Cirrus: extraction from optic disc volume scan). For the current analysis, eyes were excluded from the analysis if neither ring nor macular scan passed quality control. We further excluded data from the less common instrument for 1 center. The final cohort included 364 macular and 481 peripapillary scans of 501 eyes from 283 patients and 136 eyes from 72 HCs. Lower numbers of macular scans compared with peripapillary scans were due to both lower submission of macular data and more exclusions based on quality concerns.

Visual and Global Function Testing

High-contrast visual acuity (HC-VA) was available for 497 (99.2%) patient and 10 (13.9%) HC eyes. HC-VA was best corrected for 212 (42.3%) patient and 56 (41.2%) HC eyes, habitually corrected for 145 (28.9%) patient and 2 (<0.1%) HC eyes, and without correction for 140 (27.9%) patient eyes and 17 (1.3%) HC eyes. All VA data are reported as logMAR. VA measurement method was decided on discretion of each center. Visually evoked potentials (VEPs) were available for 167 (33.3%) patient eyes and 40 (29.4%) HC eyes, with P100 latency recorded as a binary value (normal/prolonged). Expanded Disability Status Scale (EDSS) scores were determined at the discretion of each center with data available for 180 (63.6%) patients.

Statistical Analysis

Statistical analyses were performed with R version 3.6.1 using RStudio and R Markdown (RStudio Inc., Boston, MA).16 p Values less than 0.05 were considered significant. We considered p values less than 0.10 a trend. Group matching by age and sex for confirmatory analyses was performed using automatic matching by R package MatchIt (method: exact). Group comparisons and correlations of OCT and VA values were performed using linear mixed-effect models. Intereye within-subject effects and effects of the center were included as random effects. Sex, ethnicity, and age were included as fixed effects for the analyses in the entire cohort. Age and sex were not included for the matched subset. For OCT parameters, the model was used for data from all devices separately and combined by Fisher combined probability test. The marginal and conditional coefficients of determination of the linear models were calculated with pseudo R-squared. All results are reported combined and individually for Spectralis SD-OCT; the analyses of data acquired by Cirrus HD-OCT or Topcon 3D OCT-1 and for the matched subset are reported as online-only supplement.

Data Availability

The data supporting the findings of this study are available within the article and from the corresponding author by reasonable request.

Results

Five hundred one eyes of 283 AQP4-IgG–seropositive patients and 136 eyes of 72 HCs were included in the analysis (Table 1).

Table 1.

Cohort Description for AQP4-IgG–Seropositive Patients With NMOSD and HCs

graphic file with name NEURIMMINFL2021038919t1.jpg

Neuroaxonal Damage After ON

GCIP layer and pRNFL were reduced in NMOSD-ON compared with NMOSD eyes without a history of ON (NMOSD-NON) and HC (GCIP layer: 81.4 ± 5.7 μm, pRNFL: 101.1 ± 9.0 μm) (Figures 1A and 2A; Tables 2 and 3). The absolute (GCIP layer: −22.7 μm; pRNFL: −38.5 μm) and relative (GCIP layer: −38.8%; pRNFL: −61.6%) loss in eyes with 1 ON episode (NMOSD-1-ON) compared with NMOSD-NON was higher than in eyes with 2 ON episodes (NMOSD-2-ON) compared with NMOSD-1-ON (GCIP layer absolute loss: −3.5 μm, relative loss: −6.0%; pRNFL absolute loss: −9.1 μm, relative loss: −14.5%, n.s.). The loss in eyes with ≥3-ON episodes (NMOSD-3-ON) was lower compared with NMOSD-2-ON (n.s.) (Figures 1B and 2B). Five NMOSD-NON eyes had pRNFL values < 60 μm; these patients had no relevant comorbidities but a history of contralateral ON.17,18 In sensitivity analyses to account for device differences, all significant analyses within the NMOSD cohort were confirmed for data acquired by Cirrus and Topcon OCT devices (eFigures 2 and 3; eTables 3 and 4, links.lww.com/NXI/A556; links.lww.com/NXI/A557).

Figure 1. Group Comparisons of GCIP Layer Thickness.

Figure 1

Boxplots of GCIP layer thicknesses (μm) acquired by Heidelberg SD-OCT with values of individual eyes (jitter) for (A) HC (gray/left), NMOSD-NON (dark blue/middle), NMOSD-ON (dark red/right); for (B) number of ON episodes (NMOSD-NON dark blue/left, NMOSD-1-ON light red/left-middle, NMOSD-2-ON medium-red/right-middle, NMOSD-≥3-ON medium-dark red/right); and for (C) HC (gray/left), NMOSD-NONnon (light blue/middle), NMOSD-NONcon (blue/right). (D) Forest plots for results from different OCT devices for (D.a) NMOSD-NON vs NMOSD-ON, (D.b) NMOSD-NON vs NMOSD-1-ON, and (D.c) NMOSD-1-ON vs NMOSD-2-ON (eFigure 2 and eTable 2, links.lww.com/NXI/A556 and links.lww.com/NXI/A557). GCIP = ganglion cell and inner plexiform; HC = eyes of HCs; NMOSD-NON = eyes of patients with neuromyelitis optica without a history of ON; NMOSD-NON-con = eyes of patients with neuromyelitis optica without a history of ON but a history of contralateral ON; NMOSD-NONnon: = eyes of patients with neuromyelitis optica without a history of ipsilateral or contralateral ON; NMOSD-ON = eyes of patients with neuromyelitis optica with a history of ON; NMOSD-1-ON = eyes of patients with neuromyelitis optica with a history of 1 ON episode; NMOSD-2-ON = eyes of patients with neuromyelitis optica with a history of 2 ON episodes; ON = optic neuritis.

Figure 2. Group Comparisons of pRNFL Thickness.

Figure 2

Boxplots of pRNFL thicknesses acquired by Heidelberg SD-OCT [μm] with values of individual eyes (jitter) for (A) HC (gray/left), NMOSD-NON (dark blue/middle), and NMOSD-ON (dark red/right); for (B) number of ON episodes (NMOSD-NON dark blue/left, NMOSD-1-ON light red/left-middle, NMOSD-2-ON medium-red/right-middle, and NMOSD-≥3-ON medium-dark red/right); and for (C) HC (gray/left), NMOSD-NONnon (light blue/middle), and NMOSD-NONcon (blue/right). (D) Forest plots for results from different OCT devices for (D.a) NMOSD-NON vs NMOSD-ON, (D.b) NMOSD-NON vs NMOSD-1-ON, and (D.c) NMOSD-1-ON vs NMOSD-2-ON (eFigure 3 and eTable 3, links.lww.com/NXI/A556 and links.lww.com/NXI/A557). HC = eyes of HCs; NMOSD-NON = eyes of patients with neuromyelitis optica without a history of optic neuritis; NMOSD-NON-con = eyes of patients with neuromyelitis optica without a history of optic neuritis but a history of contralateral optic neuritis; NMOSD-NONnon: = eyes of patients with neuromyelitis optica without a history of ipsilateral or contralateral optic neuritis; NMOSD-ON = eyes of patients with neuromyelitis optica with a history of optic neuritis; NMOSD-1-ON = eyes of patients with neuromyelitis optica with a history of 1 optic neuritis episode; NMOSD-2-ON = eyes of patients with neuromyelitis optica with a history of 2 optic neuritis episodes; ON = optic neuritis; pRNFL = peripapillary retinal nerve fiber layer.

Table 2.

Group Comparisons of GCIP Layer Thickness for Heidelberg SD-OCT

graphic file with name NEURIMMINFL2021038919t2.jpg

Table 3.

Group Comparisons of pRNFL Thickness With (A) Spectralis SD-OCT Data for Comparisons vs HC (B) Data From All Devices for Intrapatient Cohort Comparisons

graphic file with name NEURIMMINFL2021038919t3.jpg

Neuroaxonal Damage Without ON

NMOSD-NON eyes had a reduced GCIP layer (75.9 ± 7.7 μm, p < 0.001), but not pRNFL (95.3 ± 14.4 μm) compared with HC (GCIP layer: 81.4 ± 5.7 μm, pRNFL: 101.1 ± 9.0 μm; Figures 1A and 2A; Tables 2 and 3). By comparison, only 28 NMOSD-NON eyes (5.1%) had a GCIP layer ≤ the 5th percentile of HC. GCIP layer was also reduced in NMOSD-NON with a history of contralateral ON (72.9 ± 10.2 μm) compared with NMOSD-NON without a history of contralateral ON (77.3 ± 5.9 μm, p = 0.025, NMOSD-NONnon). This effect vanished when only considering NMOSD-NONcon with a history of 1 contralateral ON (73.6 ± 9.4 μm). However, only NMOSD-NONcon (p < 0.001) but not NMOSD-NONnon (p = 0.061) had thinned GCIP layer compared with HC (Figures 1C and 2C).

INL Changes

INL was thicker in NMOSD-ON compared with HC (39.4 ± 2.6 μm) and NMOSD-NON (eTable 4, links.lww.com/NXI/A557). Specifically, INL was thicker in eyes with 1 ON episode compared with NMOSD-NON but did not differ between eyes with different numbers of ON episodes. Also, INL did not differ between NMOSD-NONcon (38.9 ± 3.2 μm) and NMOSD-NONnon (38.5 ± 3.2 μm, p = 0.931). In all patients, INL thickness (as the dependent variable in the linear mixed model described above) was correlated with thinner GCIP layer (B = −0.11, standard error [SE] = 0.01, p < 0.001) and pRNFL (B = −0.06, SE = 0.01, p < 0.001). This correlation existed in NMOSD-ON (GCIP layer: B = − 0.08, SE = 0.03, p = 0.005; pRNFL: B = − 0.06, SE = 0.01, p < 0.001) and not present in NMOSD-NON (eFigure 4, links.lww.com/NXI/A556).

Scans of 363 NMOSD eyes were clearly suitable for MME investigations (high quality, eFigure 4C, links.lww.com/NXI/A556). MMEs were visible in 24 (6.6%) eyes of 21 patients. Twenty-three eyes (13.1%) of NMOSD-ON and 1 eye (0.5%) of NMOSD-NON (with a history of contralateral ON) were affected. The number of ON episodes did not influence the incidence of MMEs (NMOSD-1-ON N = 16 (14%), NMOSD-2-ON N = 4 (11.4%), NMOSD-≥3-ON N = 3 (11.1%)).

Including only the most frequent ethnicities in our data set (Asian and non-Hispanic White), results did not differ, and no ethnicity was singled out regarding its pattern of injury (data not shown).

OCT and Vision Loss

HC-VA was numerically reduced in NMOSD (0.25 ± 0.48) compared with HC (−0.01 ± 0.08) and known healthy reference values. NMOSD-ON also had reduced HC-VA (0.44 ± 0.58) compared with NMOSD-NON (0.04 ± 0.20, p < 0.001, p = 0.401) and HC/healthy reference populations. HC-VA was correlated with GCIP layer (B = −0.016, SE = 0.002, p < 0.001) and pRNFL thicknesses (B = −0.010, SE = 0.001, p < 0.001) in AQP4-IgG–seropositive NMOSD. AQP4-IgG–seropositive NMOSD eyes with prolonged VEP latency had a thinned GCIP layer (B = −11.647, SE = 3.628, p = 0.002) and pRNFL (B = −21.965, SE = 3.724, p < 0.001). EDSS score as a metric of global disability was inversely correlated with GCIP layer (B = −1.370, SE = 0.566, p = 0.017) and pRNFL thicknesses (B = −3.148, SE = 1.080, p = 0.004).

Discussion

Our study specifies a severe and functionally relevant decrease of GCIP layer and pRNFL in NMOSD-ON compared with NMOSD-NON and HCs in AQP4-IgG–seropositive patients. Neuroaxonal damage is particularly large from the first episode of ON, where contribution to retinal damage in subsequent episodes of ON is still considerable but smaller. In contrast to previous smaller studies, the current study ascertains GCIP layer but not pRNFL thinning in NMOSD-NON compared with HC.6,19 This effect was driven by eyes with contralateral ON and not statistically significant in eyes without a history of ON. INL was thicker in NMOSD-ON and was inversely correlated with GCIP layer. Of note, 13.1% of NMOSD-ON eyes showed MME indicative of secondary inflammatory changes.20,21 This investigation overcame limits of small samples and OCT data heterogeneity of earlier studies through use of an international consortium of NMOSD specialists. This framework substantiated the CROCTINO study—a large, multicenter, retrospective evaluation of retinal pathology in NMOSD using OCT.

The neuroaxonal degeneration in NMOSD-ON demonstrated here is substantially greater than reported changes in multiple sclerosis (MS), a common differential diagnosis.2,22 In a meta-analysis, an average pRNFL loss of 20 μm was estimated in MS after ON, which is nearly 2-fold higher in our NMOSD-ON cohort (−38.4 μm).22 For GCIP layer, our data suggested approximately 1.5-fold higher loss in NMOSD (−24.0 μm) compared with MS.22 These differences not only result from a higher ON frequency, but may also be caused by more severe retinal damage in NMOSD after a singular ON.23 It is intriguing that the damage is particularly large after the first ON episode with smaller losses after subsequent episodes, which might be due to less neuroaxonal content in subsequent episodes.2,24 Alternatively, although the analysis of treatment effects exceeds the scope of this study, it is possible that the longer time to effective anti-inflammatory therapy and the typical choice of less effective therapies (e.g., steroids instead of plasma exchange) at the first attack compared with following attacks may significantly contribute to this difference. Independent of the number of ONs, the percent loss is smaller in GCIP layer compared with pRNFL–pointing toward either (1) stronger loss of retinal nerve fibers than retinal ganglion cells, (2) impairment of ganglion cells not leading to extinction but axonal loss; (3) a larger amount of non-neuronal tissue in GCIP layer, or (4) RNFL loss in the periphery beyond the macular area measured by GCIP layer. These hypotheses are not mutually exclusive, and each might contribute to the effect. Consistent with these concepts, excessive vision loss relative to neuroaxonal content and VEP latency in AQP4-IgG–seropositive patients compared with MOG-IgG–seropositive and MS patients implies damage of the peripheral retina and optic nerve tissues, which are not reflected in the macula and pattern VEP measurements.4

Whether attack-independent neurodegeneration in AQP4-IgG–seropositive NMOSD occurs has been controversial.2,6,7,25-28 The current study identifies decreased GCIP layer in NMOSD-NON compared with HC, but not pRNFL. Such subclinical changes could be caused by contralateral involvement after unilateral ON.17,18 Indeed, our cohort suggests pronounced neurodegeneration in eyes with contralateral ON. However, eyes of patients without ON also exhibit a trend for thinner GCIP layer compared with HCs. Underscored by longitudinal studies showing ON-independent neurodegeneration and VEP latency prolongation,7,29 such patterns suggest at least 2 mechanisms of injury: (1) a primary retinopathy or optic neuropathy in context of an astrocytopathy or caused by direct damage to AQP4-expressing cells such as astrocytes and Müller cells by either AQP4-IgG or AQP4-specific T cells or (2) a global or afferent visual system specific chronic or episodic neurodegenerative process. Because lesions often spare the brain, and most studies outside of predisposed areas such as optic nerve and spinal cord failed to detect effects,30 a constant global involvement seems unlikely. Several studies described changes affecting AQP4 expression and astrocytic end feet,31 parafoveal changes in agreement with an involvement of AQP4-expressing Müller cell,6,26,27,32 and attack-independent spinal cord atrophy.33 These findings are consistent with tissue infiltration by AQP4-IgG–specific T cells34 and the attack-independent loss of retinal ganglion cells7—further supporting the existence of an ON-independent pathology, which might be restricted to the main disease foci. The latter hypotheses could be addressed by region-specific pathology or advanced imaging studies.

INL changes have been suggested as a marker of neuroinflammation and potential treatment response in MS.21,35,36 MME may develop as a consequence of neurodegeneration or other—non–disease-specific—processes.35 Patients with NMOSD were described to have INL thickening and MME.20,37 In our cohort, 13.1% of NMOSD-ON eyes were affected by MME, which is higher than the 2%–5% described in MS but comparable to incidences in NMOSD described before by Gelfand and colleagues.35,37 However, INL thickening itself remained comparable to changes reported in MS.35 This disparity could reflect a disrupted fluid homeostasis due to Müller cell involvement or loss of content of the INL with a parallel inflammatory reaction and development of MME. The limited accessibility of MMEs, especially in severely affected eyes due to limited image quality, has hindered their detailed assessments and most likely leads to underestimation of their incidence.38 We demonstrate that the INL thickness is inversely correlated with neuroaxonal content and could be a valuable marker of disease severity also in NMOSD.

Our study cohort was representative of patients with AQP4-IgG–seropositive NMOSD with respect to a female predominance and ON history.11,39 This OCT study included multiple ethnicity backgrounds, although the distribution was shifted toward Caucasian/White patients and other ethnicities were underrepresented (e.g., Hispanic White and Black), limiting the generalizability of results.40 Patients from different heritages presented similar findings.

The current study was based on source data instead of a meta-analysis. Using the Guthy-Jackson Charitable Foundation network, this multicentric study was conducted without investigator reimbursement and illustrated how collaboration integrating international perspectives can produce meaningful results. To overcome technical challenges of heterogeneous source data, we developed novel OCT postprocessing techniques allowing us to perform standardized analyses and enabling the uniform analysis of the largest OCT image data set in NMOSD to date.10 Thus, the strengths of CROCTINO include its established infrastructure, large international network of experts representing multiple ethnicities and geographic regions, and the use of state-of-the-art OCT postprocessing techniques.41

We recognize limitations of the current investigation: The retrospective and heterogeneous data acquisition might have led to biases and impreciseness beyond the ability of our quality control. We addressed this by excluding uncertain cases. HCs were only included from a limited number of centers. The unbalanced data set limited some analyses, such as the influence of ethnicities or acute and disease-modifying treatments. Case-control matching was impossible, particularly with respect to subclinical progression dependent or independent of ON and to ethnicity. Similarly, comparisons with other NMOSD subtypes or MS were beyond the scope of this study. Longitudinal data, acute ON data, and AQP4-IgG–seronegative and MOG-IgG–seropositive patient data are part of the CROCTINO data set and will be analyzed in the future. Also, OCT data processing was performed by multiple raters potentially introducing interrater variability. MRI data and posterior visual pathway involvement were not investigated in this study. However, the current study achieved an unprecedented worldwide assessment of retinal damage in AQP4-IgG–seropositive NMOSD.

To conclude, AQP4-IgG–seropositive NMOSD is characterized by a severe, functionally relevant retinal neurodegeneration as a consequence of ON. Although the majority of damage occurs during the first episode, there is cumulative loss with each succeeding relapse. The ON-associated damage is not limited to the neuroaxonal content but can also induce—likely inflammation-mediated—INL increase and occurrence of MME. Our data also suggest attack-independent retinal damage in AQP4-IgG–seropositive NMOSD. Our study supports that attack prevention is key in avoiding neuroaxonal damage and vision loss in patients with NMOSD. It further suggests that the first ON episode causes the most damage, where only some patients with then established diagnosis will be on immunosuppressive therapy. This highlights the need for effective therapies that can ameliorate an ongoing attack or regenerate attack-generated damage, which is an unmet clinical need. Last, the study emphasizes the utility of OCT as a sensitive structural metric and its potential for monitoring progression and even treatment response in AQP4-IgG–seropositive NMOSD. The international CROCTINO program provides an unprecedented opportunity to apply OCT in a standardized manner to assess pathophysiology, clinical course, and therapeutic efficacy in NMOSD.

Glossary

AQP4-IgG

aquaporin-4 IgG

CROCTINO

Collaborative Retrospective Study on retinal OCT in Neuromyelitis Optica

EDSS

Expanded Disability Status Scale

GCIP

ganglion cell and inner plexiform

HC

healthy control

HC-VA

high-contrast visual acuity

INL

inner nuclear layer

MME

microcystic macular edema

MS

multiple sclerosis

MOG-IgG

myelin oligodendrocyte glycoprotein IgG

NMOSD

neuromyelitis optica spectrum disorder

NMOSD-NON

NMOSD eyes without a history of ON

OCT

optical coherence tomography

ON

optic neuritis

pRNFL

peripapillary retinal nerve fiber layer

SE

standard error

VA

visual acuity

VEP

visually evoked potential

Appendix. Authors

Appendix.

Study Funding

Guthy-Jackson Charitable Foundation (GJCF) and German Research Foundation (DFG).

Disclosure

F.C. Oertel was employee of Nocturne GmbH and receives research support by the American Academy of Neurology and the National Multiple Sclerosis Society, unrelated to this work. S. Specovius reports no disclosures. H.G. Zimmerman reports research grants from Novartis and speaking honoraria from Bayer Healthcare, unrelated to this study. C. Chien reports speaking honoraria from Bayer Healthcare, unrelated to this work. S. Motamedi and C. Bereuter report no disclosures. L. Cook reports grants from Guthy Jackson Charitable Foundation, during the conduct of the study. E.H. Martinez-Lapiscina is employed by the European Medicines Agency (Human Medicines) since 16 April 2019. This article is related with her activity under Hospital Clinic of Barcelona/IDIBAPS affiliation and consequently, as external activity, it does not represent the views of the Agency, its Committees or working parties. Before enrolling EMA, Dr. Martinez-Lapiscina reports grants from Instituto de Salud Carlos III (Spain) & Fondo Europeo de Desarrollo Regional, grants from MS Innovation GMSI, other from Fundació Privada Cellex, and personal fees from Novartis, Roche, Sanofi-Genzyme, outside the submitted work. M.A. Lana Peixoto and M.A. Fontanelle report no disclosures. H.J. Kim reports grants from the National Research Foundation of Korea, personal fees from Alexion Pharmaceuticals, Aprilbio, Biogen, Celltrion, Eisai, HanAll BioPharma, MDimune, Merck Serono, Novartis, Sanofi Genzyme, Teva-Handok, and Viela Bio, other from Viela Bio (formerly MedImmune), Multiple Sclerosis Journal, and Journal of Clinical Neurology, outside the submitted work. J.-W. Hyun reports grants from the National Research Foundation of Korea, outside the submitted work. J. Palace reports personal fees from Abide Therapeutics, Alexion Pharmaceuticals, ARGENX, Bayer Schering, Biogen Idec, Chugai Pharma, EuroImmun, Genzyme, MedDay, MedImmune, Merck Serono, Novartis, Roche, Teva, UCB, and Viela Bio; grants from Abide Therapeutics, Alexion Pharmaceuticals, Bayer Schering, Biogen Idec, Chugai Pharma, Genzyme, MedImmune, Merck Serono, Novartis, and Teva, grants from Merck Serono, Novartis, Biogen Idec, Teva, Abide, MedImmune, Bayer Schering, Genzyme, Chugai and Alexion; Eugène Devic European Network, the Grant for Multiple Sclerosis Innovation, the John Fell Fund, the Medical Research Council, the MS Society, Myaware, the UK National Institute for Health Research, Oxford Health Services Research Committee, and the Guthy-Jackson Charitable Foundation AMPLO and SPARKS Great Ormond Street, for research studies outside the submitted work. M.I. Leite and S. Sharma report no disclosures. A. Roca-Fernandez is sponsored by Abide Therapeutic outside of the submitted work and reports no potential conflicts of interest. R. Kafieh, A. Dehghani, M. Pourazizi, L. Pandit and A. D'Cunha report no disclosures. O. Aktas reports grants from German Research Foundation (DFG) and German Ministry of Education and Research (BMBF); personal fees from Alexion and Almirall; grants and personal fees from Biogen, personal fees from Merck, grants and personal fees from Novartis, grants and personal fees from Roche, personal fees from Sanofi, personal fees from Teva, personal fees from Viela Bio, outside the submitted work. M. Ringelstein received speaker honoraria from Novartis, Bayer Vital GmbH, Roche, Alexion and Ipsen and travel reimbursement from Bayer Schering, Biogen Idec, Merz, Genzyme, Teva, Roche, and Merck, none related to this study. P. Albrecht reports grants, personal fees and non-financial support from Allergan, Biogen, Merck, Merz Pharmaceuticals, Novartis, Ipsen, Celgene, Roche, and personal fees and non-financial support from Teva outside the submitted work. E. May and C. Tongco report no disclosures. L. Leocani reports personal fees from Roche, Merck, Bristol Myers Squibb, and Med-ex learning, outside the submitted work. M. Pisa reports no disclosures. M. Radaelli reports personal fees from Merck Serono, Sanofi-Genzyme, Novartis, and Biogen, outside the submitted work. H. Stiebel-Kalish reports no disclosures. S. Siritho reports personal fees from Novartis, Thailand, Biogen Idec, Eisai Thailand marketing co. ltd., Merck Serono, Teva Thailand, and Menarini, outside the submitted work. J. de Seze and T. Senger report no disclosures. J. Havla reports grants, personal fees and non-financial support from Merck, personal fees from Novartis, Celgene, Roche, Santhera, Biogen, Alexion, Sanofi, non-financial support from Guthy-Jackson Charitable Foundation, and grants from Friedrich Baur Foundation, outside the submitted work. R. Marignier, A.C. Calvo, D. Bichuetti, I.M. Tavares, N. Asgari, K. Soelberg, A. Altintas, R. Yildirim, U. Tanriverdi, A. Jacob, S. Huda, Z. Rimler and A. Reid report no disclosures. Y. Mao-Draayer reports grants from NIH NIAID, grants and personal fees from Genzyme, grants from Chugai, personal fees from Biogen and EMD Serono, grants and personal fees from Genentech and Novartis, during the conduct of the study; grants from NIH NIAID, grants and personal fees from Genzyme, grants from Chugai, personal fees from Biogen and EMD Serono, grants and personal fees from Genentech and Novartis, outside the submitted work. I. Soto de Castillo reports no disclosures. A. Petzold reports personal fees and grants from Novartis, outside the submitted work, and is part of the steering committee of the OCTiMS study which is sponsored by Novartis. He has not received honoraria for this activity. A.J. Green reports other from Bionure, grants, personal fees and other from Inception Sciences, grants from Sherak Foundation, personal fees and other from Pipeline Pharmaceuticals, grants from Hilton Foundation, Adelson Foundation, and National MS Society, personal fees from JAMA Neurology, personal fees and other from Mediimmune/Viela, outside the submitted work. In addition, Dr. Green has a patent Small Molecule drug for Remyelination pending and has worked on testing off label compounds for remyelination. M.R. Yeaman serves as an advisor to the Guthy-Jackson Charitable Foundation. T. Smith reports no disclosures. A.U. Brandt reports grants from the Guthy Jackson Charitable Foundation during the conduct of the study; shares from Motognosis GmbH and shares from Nocturne GmbH outside the submitted work. In addition, Dr. Brandt has a patent pending describing Foveal Morphometry. F. Paul reports grants from the Guthy Jackson Charitable Foundation, during the conduct of the study; grants from the German Research Foundation and German Federal Ministry of Education and research, grants, and other from Novartis, grants and other from Bayer, Novartis, Biogen Idec, Teva, Sanofi-Aventis/Genzyme, Merck Serono, Chugai, other from PLoS ONE, other from Neurology® Neuroimmunology & Neuroinflammation, other from SanofiGenzyme, Biogen Idec, grants from German Research Council, Werth Stiftung of the City of Cologne, German Ministry of Education and Research, Arthur Arnstein Stiftung Berlin, EU FP7 Framework Program, Arthur Arnstein Foundation Berlin, National Multiple Sclerosis (USA), other from MedImmune, Shire, Alexion, outside the submitted work. In addition, Dr. Paul has a patent Foveal Morphometry pending to Nocturne GmbH. Go to Neurology.org/NN for full disclosures.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The data supporting the findings of this study are available within the article and from the corresponding author by reasonable request.


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