H. baltica hfsH mutant holdfasts forms thread-like fibers that diffuse into the medium
(A) Time-lapse montages of H. baltica WT and H. baltica ΔhfsH on soft agarose pads. Exponential cultures were placed on soft agarose pads containing holdfast-specific AF488-WGA (green) and covered with a glass coverslip. Images were collected every 5 min for 12 h. Scale bar, 2 μm.
(B) Time-lapse montages of H. baltica WT and H. baltica ΔhfsH in microfluidic channels. Exponential cultures with holdfast-specific AF488-WGA (green) were injected into the microfluidic chambers, and flow was turned off. Images were collected every 20 s for 5.5 h. Scale bar, 2 μm. See also Videos S1, S2, S3, and S4.