Skip to main content
. 2021 Sep 2;24(9):103071. doi: 10.1016/j.isci.2021.103071

Figure 7.

Figure 7

HfsH expression is required for interaction of holdfast thiols and galactose monosaccharides with cells

(A) Representative images showing merged phase and fluorescence channels of H. baltica and H. baltica ΔhfsH holdfasts colabeled with AF488-WGA (green, GlcNAc) to label polysaccharides and AF594-mal (red) to label free thiols. Scale bar, 2 μm.

(B) Representative images of H. baltica WT and H. baltica ΔhfsH complemented with pMR10:PCu-hfsH. Holdfasts were colabeled with AF488-WGA (green) and AF594-Mal (red). Exponential cultures were induced for 2 h with concentrations of CuSO4 ranging from 0–250 μM. Scale bar, 2 μm.

(C) Representative images showing merged phase and fluorescence channels of H. baltica and H. baltica ΔhfsH holdfasts colabeled with AF488-WGA (green) and AF594-GSL-1 (red) to label GlcNAc and galactose in holdfast, respectively. Scale bar, 2 μm.