Figure 2.

SSPbP53 is an apoplastic protein and inhibits apoplastic cruciferous PLCPs. A. Confocal images showing SSPbP53-GFP localized to the apoplast of rapeseed cotyledons, after salt-induced plasmolysis (Scale bars, 20 μM). Arrows indicate the retraction of the membrane from the cell wall. B. His-SSPbP53 inhibits Arabidopsis apoplastic PLCPs. Apoplastic proteins from Arabidopsis Col-0 roots were labeled via ABPP in the presence of purified His-SSPbP53. WB, western blot; CBB, coomassie brilliant blue; LC, Loading control. C. His-SSPbP53 inhibits apoplastic PLCPs in rapeseed roots. D. His-SSPbP53 transiently expressed in rapeseed cotyledons inhibits apoplastic PLCPs, A. tumefaciens containing empty vector (EV) was used as a negative control, together with non-treated cotyledons (Full figure in Supplementary Fig. S5)