Table 1.
Calculated values of Ks (in μM) and ΔA values from Soret titrations. Each of the pCBs (THC, CBD, CBG, CBC, CBDV, THCV and β-CP) were titrated against the four constraints of CYP2D6 (WT, *2, *10 and *17) The values are calculated from the means of three repeats (N=3). The error represents the standard error of the mean of three experiments.
| Cannabinoids | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| THC | CBD | CBG | CBN | CBC | CBDV | THCV | β-CP | ||
| WT | Ks (μM) | 3.41 ± 1.08 | 7.03 ± 2.24 | 13.42 ± 3.25 | 7.90 ± 2.89 | 6.09 ± 2.51 | 7.75 ± 2.76 | 3.24 ± 1.12 | 4.27 ± 1.30 |
| ΔA | 0.0486 ±.0032 | 0.0711 ±0.0060 | 0.0640 ±0.0054 | 0.0362 ±0.0038 | 0.0246 ±.0026 | 0.0217 ±0.0022 | 0.0252 ±0.0018 | 0.0281 ±0.0020 | |
| *2 | Ks (μM) | 3.46 ± 1.03 | 10.51 ± 3.67 | 10.36 ± 3.48 | 5.13 ± 0.94 | 7.94 ± 2.24 | 11.56 ± 4.36 | 11.52 ± 3.92 | 10.31 ± 4.71 |
| ΔA | 0.0142 ±0.0009 | 0.0299 ±0.0033 | 0.0377 ±0.0040 | 0.0329 ±0.0015 | 0.0377 ±0.0031 | 0.0387 ±0.0048 | 0.0392 ±0.0044 | 0.0381 ±0.0055 | |
| *10 | Ks (μM) | 8.51 ± 2.99 | 5.23 ± 2.34 | 7.28 ± 3.14 | 3.87 ± 1.26 | 9.16 ± 2.28 | 7.19 ± 2.55 | 11.50 ± 2.87 | 13.95 ± 5.27 |
| ΔA | 0.0332 ±0.0034 | 0.0320 ±0.0036 | 0.0596 ±0.0072 | 0.0203 ±0.0014 | 0.0299 ±0.0022 | 0.0385 ±0.0038 | 0.0346 ±0.0028 | 0.0297 ±0.0040 | |
| *17 | Ks (μM) | 20.10 ± 8.24 | 5.57 ± 1.22 | 11.77 ± 3.18 | 9.00 ± 2.13 | 11.64 ± 3.20 | 8.60 ± 1.86 | 7.12 ± 1.59 | 10.27 ± 3.82 |
| ΔA | 0.0737 ±0.0125 | 0.0247 ±0.0014 | 0.0745 ±0.0067 | 0.1387 ±0.0098 | 0.112 ±0.0101 | 0.162 ±0.00873 | 0.1516 ±0.0093 | 0.0809 ±0.0095 | |