Figure 2.
Adar1mZα/mZα lungs display a type I IFN gene signature
Total RNA was extracted from lungs of three WT and three Adar1mZα/mZα mice. Ribosomal RNAs were depleted before random-primed library preparation and RNA sequencing. About 100 million reads were obtained per sample.
(Α) Differentially expressed genes were defined as displaying a fold change of ≥2 with an adjusted p value < 0.01. The 89 induced and 10 repressed genes were ordered by decreasing fold change and the data were clustered by sample. ISGs are indicated in bold.
(B) GO analysis of induced genes. The top 20 GO terms (biological processes), ranked and ordered by p value, are shown. Diameters indicate the number of induced genes assigned to the GO term and colors show the p value.
(C) Detected and differentially expressed REs were assigned to the indicated classes and are shown as pie charts. Differentially expressed REs were identified as having a minimum fold change of 2 and an adjusted p value of less than 0.01.