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(A) Western blots of VCAM-1, p-IκBα, IκBα and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Western blot quantification for (B) VCAM-1 expression, (C) p-IκBα/IκBα ratio, (D) nuclear NF-κB p65, and (E) THP-1 monocyte adhesion, all for PAEC monolayers cultured in conditioned media from PAECs sheared in segmented wells, with and without added TNF-α.
