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Effect of conditioned medium on NF-κB p65 nuclear translocation in PAECs treated with TNF-α.
The medium was conditioned by PAECs sheared at the centre or the edge of the swirled well and then subject to sequential ultrafiltration so that it contained molecules (A) > 100 kDa, (B) 50–100 kDa and (C) 3–50 kDa.
