We have noticed inadvertent errors in our article published in the December 2018 issue of Aging Dis (2018 9(6):1058-1073). In Figure 3A, the images for the HA/TCP (control) and HA/TCP+lenti-NC (negative control), and in Figure 3D, the image of HA/TCP-lenti-NC for IBSP have been presented incorrectly. The Figure 4G was incorret. We have attached corrected Figure 3 and Figure 4. The errors do not change the scientific conclusions of the article. The authors apologize for the errors.
Figure 3.
miR-10b promotes the ectopic bone formation of hADSCs in vivo. (A) H&E staining was used to analyze osteoid formation in the xenografts. (B) Masson trichrome staining indicated collagen. (C) Safranin O staining revealed cartilage formation in the xenografts. (D) Immunohistochemical staining showed the expression levels of osteogenic markers in the xenografts. Black arrows represent positive signals. Scale bars: 200 μm.
Figure 4.
Downregulation of endogenous miR-10b suppresses osteogenic differentiation and enhances adipogenic differentiation. (A) miR-10b expression was determined by stem-loop RT-PCR. (B and C) ALP staining and ALP activity were performed after inhibiting miR-10b. (D) Alizarin red staining was performed on day 12. (E and F) qRT-PCR and western blot were performed to analyze the mRNA and protein levels of osteogenic-specific markers after miR-10b inhibitor transfection. (G and H) Oil red O staining and extraction were performed to detect the formation of lipid droplets on day 10 of adipogenic differentiation. (I and J) The expression of adipogenic-specific markers was analyzed by qRT-PCR and western blot. The data, normalized to GAPDH, are averages of 3 independent experiments (mean ± SD). *P<0.05; **P<0.01; ***P<0.001 compared with the control. Scale bars: 200 μm.