Table 1.

Selected studies of global epigenomic profiling and transcription factor binding specifically mediated by AML1 and AML1/ETO.

Chromatin marks	Transcription factors	Method	Cell type	Reference
Acetylation
H3K9ac, H3K14ac, H4panAc	ERG, FLI1, CBFB, HEB, RUNX1, ETO, AML1-ETO, RNAPII	ChIP-Seq	Cell lines, patient t(8;21) AML blasts and normal CD34+ hematopoietic cells	Martens et al. [66]
H3ac	AML1/ETO, AML1, LMO2, Pol II, Pu.1, C/EBPα, HDAC2, P300	ChIP-Seq DNAseI Footprinting	Cell lines, primary cells, normal. CD34+	Ptasinska et al. [58]
H3K9ac	AML1/ETO, AML1, Pol II	ChIP-Seq DNAse I hypersensitivity	Primary cells and cell lines	Ptasinska et al. [67]
H3K27ac	AML1/ETO	ChIP-Seq DNAse I hypersensitivity	Cell lines, primary AML cells, AML1/ETO-transduced iPSCs	Mandoli et al. [70]
H2A.Zac	AML1/ETO, P300	Nuclease accessibility coupled with high-throughput sequencing (NA-seq) and ChIP-seq	Cell lines and primary patient blasts	Saeed et al. [69]
K43ac	AML1/ETO, P300	ChIP-Seq	Cell lines	Wang et al. [36]
H3K9/14ac	AML1/ETO9a, PRMT1	ChIP-qPCR	Cell lines	Shia et al. [39]
H4ac loss	AML1/ETO, SP1	ChIP-chip analysis	Transduced human HSPCs	Maiques-Diaz [118]
Methylation
H3K4me3, H3K4me1, H3K36me3, H3K27me3, H3K9me3	AML1/ETO	ChIP-seq, DNA hypersensitivity	Cell lines, primary AML cells, AML1/ETO-transduced iPSCs	Mandoli et al. [70]
H3K4me3, H3K27me3	AML1, NCoR, P300	ChIP-Seq	Cell lines	Trombly et al. [72]
H4R3me2a	AML1/ETO9a, PRMT1	ChIP-qPCR	Cell lines	Shia et al. [39]
H3K4me3	AML1, AML1/ETO and HEB	ChIP-qPCR	Cell lines	Gardini et al. [73]
K43me	AML1/ETO, EZH1	ChIP-Seq	Cell lines	Dou et al. [38]

The chromatin marks in Wang and Dou et al. refer to site-specific lysine acetylation/methylation of the respective target protein.