Fig. 1.

Identification of the adMSCs and the Exo. a Expression of the MSC-surface positive marker proteins CD29, CD44, and CD99 and the negative marker protein CD45 in the acquired adMSCs examined by flow cytometry; b Osteogenic differentiation and adipogenic differentiation potentials of the adMSCs examined by alizarin red staining and Oil red O staining, respectively; c Morphology of the extracted particles observed under the TEM; d Particle size distribution examined by a NTA; e Expression of the exosome surface marker proteins determined by western blot analysis; f Uptake of exosomes by cardiomyocytes observed by the PHK26 labeling. Three independent experiments were performed. Representative images are presented.