Figure 5.

Vein diameter is increased in PIRKO. (A) Retinas at P5, P10 and P15 were whole-mounted and stained with isolectin B4 to assess vein diameter, scale bar 200 µm. (B) Vein diameter is increased in PIRKO at P5 and normalizes by P15. (C) Veins at P5, P10, and P15 were divided into 3 (2 at P5) equally long segments (center, middle, periphery) and proportion of segments <25 µm, between 25 and 35 µm, and >35 µm were determined. P5 retinas show an increase in larger segment vessel in PIRKO, which normalizes by P15. Data presented as mean ± SEM, unpaired t-test (B) or 2-way ANOVA with Sidak’s multiple comparison test (C), *P < .05, **P < .01, n = 9, 11 at P5, n = 7, 9 at P10, n = 7, 8 at P15; A, artery; V, vein.