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. 2021 Aug 31;10(9):2268. doi: 10.3390/cells10092268

Figure 5.

Figure 5

PR619 reduces in vitro cell proliferation. (A) Three OS cell lines (HOS, MG63 and U2OS) were treated or not for 48 h with PR619 (1, 2.5, 5, 10 or 25 μM). Cell viability was evaluated as described in the Materials and Methods section using crystal violet staining. For each cell line, the graph indicates the relative cell number compared with untreated cells. The mean ± SD of at least four independent experiments, each performed in sextuplicate, is presented (* p < 0.05, ** p < 0.01). (B) Three OS cell lines (HOS, MG63 and U2OS) were treated or not for 14 h, 24 h or 48 h with 10 μM PR619. Cell viability was evaluated as described in the Materials and Methods section using crystal violet staining. For each cell line, the graph indicates the relative cell number compared to untreated cells. The mean ± SD of at least four independent experiments, each performed in sextuplicate, is presented (* p < 0.05, ** p < 0.01).