Table 1.
The protocol of qPCR.
| Temperature | Time | |
|---|---|---|
| Enzyme activation | 95 °C | 5 min |
| 50 cycles | 95 °C | 15 s |
| 56 °C | 20 s | |
| 72 °C | 40 s | |
| Final extension | 72 °C | 5 min |
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