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. 2021 Sep 17;11(9):1373. doi: 10.3390/biom11091373

Figure 1.

Figure 1

Effects of metformin and 3-OBA on cell proliferation and metabolism. (A) RKO and HT29 cells were treated with various concentrations of metformin (5 mM, 15 mM, 45 mM) for 48 h. Cell viability was measured by CCK-8 assay. (B) GPR81 expression of a wide variety of cancers in TIMER. (C) Relative mRNA expression of GPR81 in RKO and HT29 cells. (D) RKO and HT29 cells were treated with different concentrations of 3-OBA (10 mM, 15 mM, 20 mM) for 48 h. Cell viability was measured by CCK-8 assay. (E,F) Extracellular acidification rate (ECAR) of RKO (E) or HT29 (F) cells pretreated with 15 mM 3-OBA was detected in the presence of glucose, oligomycin (OM), and 2-deoxy glucose (2-DG). Representative histograms of at least three independent experiments are shown. * p < 0.05, ** p < 0.01, *** p < 0.001.