Figure 2.

Effect of siRNAs on the expression of OX1R at the protein and mRNA levels in mouse GCs. (A–C) The protein and mRNA expression levels of OX1R were measured using Western blotting and RT-qPCR in siRNA-transfected mouse GCs. The values show the expression levels of OX1R relative to β-actin (RT-qPCR) and GAPDH (Western blotting). The data from three independent experiments were presented as the means ± SEM. Different superscripts ^a,b,c,d represent the level of significance between NC and siRNAs (p < 0.05). OX1R, orexin receptor 1; GC, granulosa cells; siOX1R, orexin receptor 1 siRNA; NC, negative control; siRNA, short interfering RNA.