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. 2021 Sep 16;26(18):5635. doi: 10.3390/molecules26185635

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Knockdown of OX1R altered the expression of the key apoptotic regulator genes in mouse GCs. (A) Relative protein expression levels of Bax, caspase-3, Bcl-2, and TNF-α measured by Western blotting in mouse GCs after siOX1R transfection for 48 h. (B) Relative mRNA expression levels of Bax, caspase-3, and Bcl-2 detected by RT-qPCR in mouse GCs after siOX1R transfection for 48. The values show the expression levels of TNF-α, caspase-3, Bax, and Bcl-2 relative to β-actin (RT-qPCR) and GAPDH (Western blotting), and the data are shown as the means ± SEM of three independent experiments (** p < 0.01, *** p < 0.001). NC, negative control; OX1R, orexin receptor type 1; GCs, granulosa cells; siOX1R, orexin receptor type 1 siRNA.

Knockdown of OX1R altered the expression of the key apoptotic regulator genes in mouse GCs. (A) Relative protein expression levels of Bax, caspase-3, Bcl-2, and TNF-α measured by Western blotting in mouse GCs after siOX1R transfection for 48 h. (B) Relative mRNA expression levels of Bax, caspase-3, and Bcl-2 detected by RT-qPCR in mouse GCs after siOX1R transfection for 48. The values show the expression levels of TNF-α, caspase-3, Bax, and Bcl-2 relative to β-actin (RT-qPCR) and GAPDH (Western blotting), and the data are shown as the means ± SEM of three independent experiments (** p < 0.01, *** p < 0.001). NC, negative control; OX1R, orexin receptor type 1; GCs, granulosa cells; siOX1R, orexin receptor type 1 siRNA.