Table 3.
EMSA probe of DNA–Snail binding reaction.
Biotin-Labeled Oligonucleotide Probe | ||
FN | F: FN-585~-560 | 5′CCCCT TCGCT TCACA CAAGT CCAGC3′ |
R: FN-585~-560 | 5′GCTGG ACTTG TGTGA AGCGA AGGGG3′ | |
LEF | F: LEF-1297~-1272 | 5′CACAC CACAC TCACA CACCC CAAAA3′ |
R: LEF-1297~-1272 | 5′TTTTG GGGTG TGTGA GTGTG GTGTG3′ | |
COX2 | F: COX2-975~-950 | 5′CCCGT GGAGC TCACA TTAAC TATTT3′ |
R: COX2-975~-950 | 5′AAATA GTTAA TGTGA GCTCC ACGGG3′ | |
COL1A1 | F: COL1A1-1080~-1055 | 5′TCACC AATGA TCACA GGCCT CCCAC3′ |
R: COL1A1-1080~-1055 | 5′GTGGG AGGCC TGTGA TCATT GGTGA3′ | |
Unlabeled Oligonucleotide Competition Probe | ||
FN | F: FN-Snail wild | 5′CCT TCGCT TCACA CAAGT CC3′ |
R: FN-Snail wild | 5′GG ACTTG TGTGA AGCGA AGG3′ | |
F: FN-Snail mutant | 5′CCT TCGCT TTGTA CAAGT CC3′ | |
R: FN-Snail mutant | 5′GG ACTTG TACAA AGCGA AGG3′ | |
LEF | F: LEF-Snail wild | 5′CAC CACAC TCACA CACCC CA3′ |
R: LEF-Snail wild | 5′TG GGGTG TGTGA GTGTG GTG3′ | |
F: LEF-Snail mutant | 5′CAC CACAC TTGTA CAAGT CC3′ | |
R: LEF-Snail mutant | 5′TG GGGTG TACAA GTGTG GTG3′ | |
COX2 | F: COX2-Snail wild | 5′GTG GAGC TCACA TTAAC TAT3′ |
R: COX2-Snail wild | 5′ATA GTTAA TGTGA GCTC CAC3′ | |
F: COX2-Snail mutant | 5′GTG GAGC TTGTA TTAAC TAT3′ | |
R: COX2-Snail mutant | 5′ATA GTTAA TACAA GCTC CAC3′ | |
COL1A1 | F: COL1A1-Snail wild | 5′CCA ATGA TCACA GGCCT CCC3′ |
R: COL1A1-Snail wild | 5′GGG AGGCC TGTGA TCAT TGG3′ | |
F: COL1A1-Snail mutant | 5′CCA ATGA TTGTA GGCCT CCC3′ | |
R: COL1A1-Snail mutant | 5′GGG AGGCC TACAA TCAT TGG3′ |