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. 2021 Sep 17;9(9):1252. doi: 10.3390/biomedicines9091252

Figure 8.

Figure 8

The NLRP3 inflammasome was activated in the CA3 of hippocampus of CKD mice, which was mitigated by AST-120 administration. We performed immunofluorescent staining against NLRP3 and ASC (apoptosis-associated speck-like protein containing caspase-associated recruitment domain) to examine NLRP3 inflammasome activation in the hippocampus of CKD mice, which revealed an increase and colocalization of NLRP3 (green) and ASC (red) foci in the CA3 area of hippocampus of CKD mice, which was effectively decreased by AST-120 administration (Panel (A)). The quantitative results of immunostaining images suggested that decreasing IS levels could reduce NLRP3 inflammasome activation in the hippocampus of CKD mice (Panel (B)). (Scale bar 50 μm; *** p ≤ 0.00; n = 3 for immunohistochemistry, 6 images were taken from each animal).