Figure 2.

Changes in plasma metabolome in response to an acute bout of ice-water swimming characterized by (A) a volcano plot of the changed metabolites analyzed by Wilcoxon matched pairs signed-rank test with Benjamini–Hochberg correction and (B) analysis of the metabolic groups’ taxonomy enrichment of the increased (upper panel) and decreased metabolites (lower panel) based on HMDB taxonomy, performed by enrichment analysis (MBROLE 2.0) with minimum of 3 metabolites per group. Size of the tiles represents the relative percentage of matched metabolites in group, the shade of the tiles represents the significance of the enrichment (FDR-adjusted p values) and (C) analysis of ice-water swimming-induced changes in amino acids (AA) and their derivates.